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AB175819

8 isoprostane ELISA Kit

5

(1 Review)

|

(23 Publications)

8 isoprostane ELISA Kit is a competitive ELISA designed to quantify 8 isoprostane with a sensitivity of 1 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.005 - 5 ng/ml
- Cited in over 20 publications
1 Images
Competitive ELISA - 8 isoprostane ELISA Kit (AB175819)
  • cELISA

Supplier Data

Competitive ELISA - 8 isoprostane ELISA Kit (AB175819)

Example of standard curve obtained with ab175819.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture extracts, Tissue, Serum

Assay type

Competitive

Sensitivity

= 1 pg/mL

Range

0.005 - 5 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's 8 isoprostane competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 8 isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8 isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model. In addition, plasma 8 isoprostane levels were found to be elevated in elderly subjects with severe hypertension and in urine from subjects with high fat diet-induced liver steatosis.

This kit can be used for the determination of 8 isoprostane in diluted urine, serum, plasma, cells and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.75" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.43" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab175819-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"10X Sample Dilution Buffer", "number":"AB175819-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"HRP Buffer", "number":"AB175819-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Wash Buffer Solution", "number":"AB175819-CMP07", "productcode":"" }, { "size":"1 x 12 µL", "name":"1,000X 8-isoprostane HRP Conjugates", "number":"AB175819-CMP02", "productcode":"" }, { "size":"1 x 2 µL", "name":"8-isoprotane Standard", "number":"AB175819-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated 96-Well Microplate", "number":"AB175819-CMP01", "productcode":"" }, { "size":"1 x 22 mL", "name":"TMB Substrate", "number":"AB175819-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

8-Isoprostane also known as F2-isoprostane is a biomarker used to measure oxidative stress in biological systems. It is a product of lipid peroxidation specifically formed from the non-enzymatic oxidation of arachidonic acid a fatty acid found in the phospholipids of cell membranes. The molecular mass of 8-isoprostane is approximately 352 grams per mole. Researchers commonly find 8-isoprostane in urine and plasma and it is also detected in tissues where oxidant injury is present.
Biological function summary

8-isoprostane is associated with damage signaling due to oxidative stress. It does not form part of larger protein complexes but exists as a standalone molecule derived from lipid peroxidation processes. 8-isoprostane affects the body's responses to oxidative damage and inflammation. It serves as an accurate marker for oxidative stress levels in various conditions due to its relative stability compared to other oxidative stress markers.

Pathways

8-isoprostane plays a role in pathways involving oxidative stress and inflammation. Although it is not part of enzymatic pathways its presence indicates the activation of cellular pathways responding to oxidative damage. 8-isoprostane relates to proteins like cyclooxygenase which also participates in processes connected to inflammation. These pathways highlight the importance of 8-isoprostane in signaling for cellular responses to oxidative injury.

8-isoprostane serves as a marker for conditions involving oxidative stress such as cardiovascular disease and chronic obstructive pulmonary disease (COPD). Its levels correlate with the extent of oxidative damage in these diseases. The assessment of 8-isoprostane can indirectly link to other proteins like protein kinase C which also participates in pathological processes related to oxidative stress and inflammation. This connection makes it a valuable tool in studying the etiology and progression of diseases where oxidative stress plays a significant role.
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Product protocols

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Target data

See full target information 1-Heptyl-1-octylcyclopentane
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Acta bio-medica : Atenei Parmensis 94:e2023015 PubMed37092629

2023

Oridonin supplementation in rabbits may help to improve the outcome of induced atherosclerosis via autophagy induction.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sultan,Bassim Mohammad,Najah Hadi,Azhar Al-Shaibany,Anil Philip,Dina Jamil,Hayder Al-Aubaidy

Antioxidants (Basel, Switzerland) 12: PubMed36671026

2023

Prevalence of Hypertension and Obesity: Profile of Mitochondrial Function and Markers of Inflammation and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés García-Sánchez,Luis Gómez-Hermosillo,Jorge Casillas-Moreno,Fermín Pacheco-Moisés,Tannia Isabel Campos-Bayardo,Daniel Román-Rojas,Alejandra Guillermina Miranda-Díaz

Cureus 14:e31504 PubMed36532904

2022

Preliminary Effects of Osteopathic Manipulative Medicine on Reactive Oxygen Species in Parkinson's Disease: A Randomized Controlled Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

James Docherty,Joerg R Leheste,Jayme Mancini,Sheldon Yao

Frontiers in nutrition 9:1016310 PubMed36407519

2022

Evaluation of pre-workout and recovery formulations on body composition and performance after a 6-week high-intensity training program.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah E Cabre,Amanda N Gordon,Noah D Patterson,Abbie E Smith-Ryan

Environmental research 216:114572 PubMed36244444

2022

Oxidative stress response in pulmonary cells exposed to different fractions of PM from urban, traffic and industrial sites.

Applications

Unspecified application

Species

Unspecified reactive species

Lamia Moufarrej,Anthony Verdin,Fabrice Cazier,Frédéric Ledoux,Dominique Courcot

International journal of molecular sciences 23: PubMed35886939

2022

Sphingolipid Catabolism and Glycerophospholipid Levels Are Altered in Erythrocytes and Plasma from Multiple Sclerosis Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Albena Momchilova,Roumen Pankov,Alexander Alexandrov,Tania Markovska,Stefan Pankov,Plamen Krastev,Galya Staneva,Evgenia Vassileva,Nikolai Krastev,Adriana Pinkas

International journal of molecular sciences 23: PubMed35457157

2022

Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Raghd Abu Helal,Harrison T Muturi,Abraham D Lee,Wei Li,Hilda E Ghadieh,Sonia M Najjar

Genes 13: PubMed35327975

2022

Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Evke,Qishan Lin,Juan Andres Melendez,Thomas John Begley

Biological & pharmaceutical bulletin 44:1254-1262 PubMed34471054

2021

Betanin Prevents Experimental Abdominal Aortic Aneurysm Progression by Modulating the TLR4/NF-κB and Nrf2/HO-1 Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Renfeng Qiu,Shuxiao Chen,Fang Hua,Shuai Bian,Jianfeng Chen,Gang Li,Xuejun Wu

Nutrients 13: PubMed33375429

2020

Improvement of Executive Function after Short-Term Administration of an Antioxidants Mix Containing Bacopa, Lycopene, Astaxanthin and Vitamin B12: The BLAtwelve Study.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Crosta,Amanda Stefani,Francesco Melani,Paolo Fabrizzi,Andrea Nizzardo,Davide Grassi,Raffaella Bocale,Stefano Necozione,Francesca Lombardi,Vanessa Castelli,Arrigo F G Cicero,Annamaria Cimini,Claudio Ferri,Giovambattista Desideri
View all publications
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