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AB287788

Adrenaline ELISA Kit

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(3 Publications)

Adrenaline ELISA Kit is a competitive ELISA designed to quantify Adrenaline with a sensitivity of 4.688 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 7.813 - 500 pg/ml
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Functional Studies - Adrenaline ELISA Kit (AB287788)
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Supplier Data

Functional Studies - Adrenaline ELISA Kit (AB287788)

Adrenaline ELISA Kit.

Typical standard curve using ab287788. A new standard curve will need to be run with every experiment.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Assay type

Competitive

Sensitivity

< 4.688 pg/mL

Range

7.813 - 500 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Adrenaline ELISA kit, ab287788, (previously known as Adrenaline/Epinephrine ELISA kit, E4359) is a competitive ELISA assay for the quantitative measurement of Adrenaline in serum, plasma, and cell culture supernatants. The density of color is inversely proportional to the amount of Adrenaline captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called E4359 Adrenaline/Epinephrine (EPI) ELISA Kit. E4359-100 is the same size as the 96 test size of ab287788.

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Recovery

[ { "sample": "Serum", "range": "85 - 105 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "89 - 105 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "95 - 100 %", "average": "= 98" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab287788-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB287788-CMP05", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate sealers", "number":"AB287788-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB287788-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB287788-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB287788-CMP01", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB287788-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB287788-CMP08", "productcode":"" }, { "size":"1 x 60 µL", "name":"Biotin- Detection antibody (Concentrated)", "number":"AB287788-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB287788-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB287788-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB287788-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenaline also known as epinephrine is a hormone and neurotransmitter with a molecular weight of approximately 183.2 g/mol. It is produced mainly in the adrenal medulla and released into the bloodstream during stress or low blood glucose levels. Adrenaline plays a mechanical role by binding to adrenergic receptors triggering a series of intracellular events that lead to physiological responses such as increased heart rate muscle strength blood pressure and sugar metabolism.
Biological function summary

Adrenaline acts as a critical mediator of the fight-or-flight response increasing energy availability and redirecting blood flow to essential organs. It operates independently rather than as part of a complex. Its biological effects also extend to the central nervous system where it modulates attention and arousal levels. This systemic regulation is essential for the body to respond swiftly to external stressors by mobilizing energy reserves and enhancing physical capabilities.

Pathways

Adrenaline exerts influence in important signaling routes such as the adrenergic receptor pathway and the cAMP signaling pathway. Through these pathways it interacts with proteins like adenylate cyclase and protein kinase A which help propagate the signal within the cell leading to various physiological effects. These pathways help the organism maintain homeostasis during periods of stress by rapidly adjusting metabolic and cardiovascular functions.

Adrenaline is implicated in the management of conditions like asthma and cardiac arrest. During an asthma attack overproduction or heightened sensitivity to adrenaline can exacerbate symptoms by causing bronchial muscles to tighten. In cardiac arrest adrenergic drugs are administered to stimulate the heart. Adrenaline levels can be influenced by other proteins such as beta-adrenergic receptors which are targets for drugs that treat these conditions by modulating adrenaline's effects on the cardiovascular and respiratory systems.
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Product protocols

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Target data

See full target information Adrenaline
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eado1533 PubMed39151008

2024

Epinephrine promotes breast cancer metastasis through a ubiquitin-specific peptidase 22-mediated lipolysis circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzhang Zhou,Peng Chu,Ya Wang,Na Li,Qiong Gao,Shengnan Wang,Juncheng Wei,Guoqing Xue,Yue Zhao,Huijun Jia,Jiankun Song,Yue Zhang,Yujie Pang,Houyu Zhu,Jia Sun,Suxian Ma,Chen Su,Bingjin Hu,Zhuoyue Zhao,Hui Zhang,Janice Lu,Jian Wang,Hongjiang Wang,Zhaolin Sun,Deyu Fang

Psychophysiology 61:e14489 PubMed38095293

2023

Effects of resistance exercises on inhibitory control and plasma epinephrine levels: A registered report of a crossover randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Lin,Hao-Chien Cheng,Yi-Luen Tsai,Hung-Wen Liu,Tsung-Min Hung

European journal of applied physiology 123:2073-2085 PubMed37199760

2023

Carbohydrate, but not fat, oxidation is reduced during moderate-intensity exercise performed in 33 vs. 18 °C at matched heart rates.

Applications

Unspecified application

Species

Unspecified reactive species

Thanchanok Charoensap,Andrew E Kilding,Ed Maunder
View all publications
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websiteProtocolBooklet
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