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Aflibercept ELISA Kit is a sandwich ELISA designed to quantify Aflibercept with a sensitivity of 30 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 30 - 1000 ng/mL

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Images

Sandwich ELISA - Aflibercept ELISA Kit (AB282906), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
30 - 1000 ng/mL
Sensitivity
= 30 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

What's included?

1 x 96 Tests
Components
Aflibercept Standard S1
1 x 300 µL
Aflibercept Standard S2
1 x 300 µL
Aflibercept Standard S3
1 x 300 µL
Aflibercept Standard S4
1 x 300 µL
Aflibercept Standard S5
1 x 300 µL
Aflibercept Standard S6
1 x 300 µL
Aflibercept Standard S7
1 x 300 µL
Assay Buffer
1 x 50 mL
HRP-conjugate Probe
1 x 12 mL
Plate sealers
2 x 1 Unit
Reactant for Aflibercept coated microtitre plate
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB substrate
1 x 12 mL
Wash Buffer (20X)
1 x 50 mL

Recommended products

Aflibercept ELISA Kit is a sandwich ELISA designed to quantify Aflibercept with a sensitivity of 30 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 30 - 1000 ng/mL

Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
30 - 1000 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 30 ng/mL

Precision

Intra assay

Sample
Overall
n
0
C.V.
< 30

Inter assay

Sample
Overall
n
0
C.V.
< 30

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Note: Aflibercept ELISA kit ab282906 is intended for Research Use Only, not for use in diagnostic procedures

Aflibercept ELISA kit is a sandwich ELISA assay for the quantification of Aflibercept concentration in human serum or plasma with high sensitivity and reproducibility. Standards and samples (serum or plasma) are added in the microtiter plate coated with the reactant for Aflibercept. After incubation, the wells are washed. The HRP conjugated probe is added and binds to aflibercept captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of aflibercept in the sample or standard. Results of samples can be determined directly using the standard curve.

Cross Reactivity: Except for Aflibercept, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.

This product is manufactured by BioVision, an Abcam company and was previously called E4854 BioSim™ Aflibercept (Eylea®)(Human) ELISA Kit. E4854-100 is the same size as the 1 x 96 tests size of ab282906.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Aflibercept ELISA Kit (ab282906), expandable thumbnail

    Sandwich ELISA - Aflibercept ELISA Kit (ab282906)

    Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com