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AB108795

Albumin Pig ELISA Kit

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(1 Publication)

Albumin Pig ELISA Kit is a Competitive ELISA for the measurement of Albumin Pig in Pig in Biofluids samples.

View Alternative Names

Albumin, ALB

1 Images
Competitive ELISA - Albumin Pig ELISA Kit (AB108795)
  • cELISA

Supplier Data

Competitive ELISA - Albumin Pig ELISA Kit (AB108795)

Representative Standard Curve using ab108795

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Pig

Assay type

Competitive

Sensitivity

= 2.3 µg/mL

Range

3.13 - 100 µg/mL

Assay time

3h

Assay Platform

Microplate

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Albumin Pig in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in plasma and serum.

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108795-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108795-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108795-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Microplate (12 x 8 well strips)", "number":"AB108795-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108795-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108795-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Standard", "number":"AB108795-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108795-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108795-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated Pig Albumin (Lyophilized)", "number":"AB108795-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
See full target information ALB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2018:1078547 PubMed29577046

2018

Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Montanari,Joel Pimenta,Luca Szabó,François Noverraz,Solène Passemard,Raphael P H Meier,Jeremy Meyer,Jonathan Sidibe,Aurelien Thomas,Henk-Jan Schuurman,Sandrine Gerber-Lemaire,Carmen Gonelle-Gispert,Leo H Buhler
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websiteProtocolBooklet
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Product promise

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