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AB108793

Albumin Rabbit ELISA Kit

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(1 Publication)

Albumin Rabbit ELISA Kit is a competitive ELISA used to quantify Rabbit Albumin with a sensitivity of 0.15 µg/mL.

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including serum, plasma, and urine
- Wide dynamic range – quantifies 0.195 - 200 µg/mL

View Alternative Names

Albumin, ALB

1 Images
Competitive ELISA - Albumin Rabbit ELISA Kit (AB108793)
  • cELISA

Supplier Data

Competitive ELISA - Albumin Rabbit ELISA Kit (AB108793)

Representative standard curve using ab108793

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Rabbit

Assay type

Competitive

Sensitivity

>= 0.15 µg/mL

Range

0.195 - 200 µg/mL

Assay time

3h

Assay Platform

Microplate

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Albumin Rabbit ELISA Kit ab108793 is a competitive ELISA kit to measure Rabbit Albumin in Serum, Plasma, and Urine with a sensitivity of 0.15 µg/mL.

How the assay works

Rabbit Albumin ELISA Kit ab108793 employs an Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Strepatvidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Strepatvidin-Peroxidase enzymatic reaction. TMB is catalyzed by Strepatvidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

Assay Specificity

This kit recognizes both native rabbit albumin in serum, plasma, and urine samples.

Albumin Rabbit ELISA Kit ab108793 protocol summary:

1. Add standard or sample to each well used and add prepared biotin protein to each well. Incubate at room temperature.
2. Wash each well and add Streptavidin Conjugate Solution. Incubate at room temperature.
3. Add TMB Solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.

How other researchers are using Albumin Rabbit ELISA Kit ab108793

Albumin Rabbit ELISA Kit ab108793 has been used to study the contribution of renal nerves in rabbit models for chronic kidney disease.

References:
(1) Y. Sata, et.al., 2020, PMID: 32895021

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108793-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108793-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Standard", "number":"AB108793-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Microplate (12 x 8 well strips)", "number":"AB108793-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108793-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108793-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108793-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108793-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108793-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2X Biotinylated Albumin", "number":"AB108793-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
See full target information Albumin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hypertension (Dallas, Tex. : 1979) 76:1470-1479 PubMed32895021

2020

Contribution of the Renal Nerves to Hypertension in a Rabbit Model of Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Sata,Sandra L Burke,Cindy Gueguen,Kyungjoon Lim,Anna M D Watson,Jay C Jha,Nina Eikelis,Kristy L Jackson,Gavin W Lambert,Kate M Denton,Markus P Schlaich,Geoffrey A Head
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websiteProtocolBooklet
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Product promise

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