Albumin Rabbit ELISA Kit ab108793 is used to quantify Albumin Rabbit with a sensitivity of 1.1 ng/ml.
Competitive ELISA Kit
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- Hypertension (Dallas, Tex. : 1979) 76:1470-14792020Contribution of the Renal Nerves to Hypertension in a Rabbit Model of Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Sata et. al.PubMed 32895021
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Rabbit
- Range
- 0.195 - 200 µg/mL
- Assay time
- 3h
- Sensitivity
- >= 0.15 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
Alternative names
Albumin, ALB
What's included?
Recommended products
Albumin Rabbit ELISA Kit ab108793 is used to quantify Albumin Rabbit with a sensitivity of 1.1 ng/ml.
Competitive ELISA Kit
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Rabbit
- Range
- 0.195 - 200 µg/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- >= 0.15 µg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 6.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 11.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Albumin rabbit in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rabbit Albumin in plasma, serum, urine, cell culture, cell lysate, and tissue samples.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Strepatvidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Strepatvidin-Peroxidase enzymatic reaction. TMB is catalyzed by Strepatvidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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Competitive ELISA - Albumin Rabbit ELISA Kit (ab108793)
Representative standard curve using ab108793
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