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AB108672

Androstenedione ELISA Kit

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(5 Publications)

Androstenedione ELISA Kit is a competitive ELISA designed to quantify Androstenedione with a sensitivity of 0.01 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.1 - 10 mg/mL
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Competitive ELISA - Androstenedione ELISA Kit (AB108672)
  • cELISA

Unknown

Competitive ELISA - Androstenedione ELISA Kit (AB108672)

Representative Standard Curve using ab108672

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Assay type

Competitive

Sensitivity

= 0.01 ng/mL

Range

0.1 - 10 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.

A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108672-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108672-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 1 – 0.1 ng/mL", "number":"AB108672-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foils", "number":"AB108672-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108672-CMP14", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Androstenedione IgG Coated Microplate (12 x 8 wells)", "number":"AB108672-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Washing Solution", "number":"AB108672-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 5 – 10.0 ng/mL", "number":"AB108672-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Control", "number":"AB108672-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 0 – 0 ng/mL", "number":"AB108672-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 3 – 1.2 ng/mL", "number":"AB108672-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 4 – 4.0 ng/mL", "number":"AB108672-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"Androstenedione Standard 2 – 0.4 ng/mL", "number":"AB108672-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108672-CMP11", "productcode":"" }, { "size":"1 x 21 mL", "name":"Androstenedione-HRP Conjugate", "number":"AB108672-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Androstenedione also known as 4-androstenedione is a steroid hormone with a molecular weight of approximately 290.44 g/mol. It serves as an important intermediate in the biosynthesis of androgens and estrogens. This hormone predominantly expresses in the adrenal glands ovaries and testes where it plays a significant role in the production of sex steroids. Androstenedione circulates in the blood and can be converted into testosterone and estrone by enzymes present in specific tissues.
Biological function summary

Androstenedione acts as a precursor for the synthesis of potent androgens and estrogens contributing to the development and maintenance of secondary sexual characteristics. It is not part of a larger protein complex operating instead through enzyme-mediated conversions. These include the aromatization to form estrone and the reduction to produce testosterone. These biochemical transformations influence physiological processes such as reproductive function and secondary sexual expression.

Pathways

Androstenedione participates in the steroidogenesis pathway which is essential for the production of steroid hormones. Cholesterol provides the initial substrate for this pathway which then leads to the formation of androstenedione through a series of enzymatic reactions. The cytochrome P450 enzyme family particularly CYP17A1 plays an important role in generating androstenedione from its precursors. Additionally it links to the estrogen biosynthesis pathway interacting with enzymes such as aromatase that convert androstenedione to estrone.

Androstenedione associates with conditions like polycystic ovary syndrome (PCOS) and adrenal hyperplasia. Both disorders involve dysregulation of steroid hormone production and result in elevated levels of androgenic hormones. In PCOS increased androstenedione levels contribute to symptoms such as hirsutism and infertility. Related proteins such as 17-beta-hydroxysteroid dehydrogenase type 3 also interact with androstenedione during the manifestation of these diseases. Understanding the role of androstenedione in these contexts aids in developing therapeutic strategies to manage hormone-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Androstenedione
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Andrologia 54:e14617 PubMed36257721

2022

Are androstenedione, dihydrotestosterone, thyroid-stimulating hormone, insulin-like growth factor I, and insulin-like growth factor binding protein 3 necessary for isolated micropenis healthy boys' evaluation without any phenotypic abnormalities? A cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

Soheila Siroosbakht,Sadra Rezakhaniha,Bijan Rezakhaniha

Ecotoxicology and environmental safety 241:113741 PubMed35679729

2022

Chronic dietary exposure to a glyphosate-based herbicide results in reversible increase early embryo mortality in chicken.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Estienne,Mathias Fréville,Guillaume Bourdon,Christelle Ramé,Joël Delaveau,Christophe Rat,Marine Chahnamian,Aurélien Brionne,Pascal Chartrin,Hans Adriensen,François Lecompte,Pascal Froment,Joëlle Dupont

Clinical cancer research : an official journal of the American Association for Cancer Research 27:3980-3989 PubMed34016642

2021

Steroid Ligands, the Forgotten Triggers of Nuclear Receptor Action; Implications for Acquired Resistance to Endocrine Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Bleach,Stephen F Madden,James Hawley,Sara Charmsaz,Cigdem Selli,Katherine M Sheehan,Leonie S Young,Andrew H Sims,Pavel Souček,Arnold D Hill,Marie McIlroy

Experimental biology and medicine (Maywood, N.J.) 243:695-707 PubMed29763383

2018

EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Jj Ronis,Horacio Gomez-Acevedo,Kartik Shankar,Neha Sharma,Michael Blackburn,Rohit Singhal,Kelly E Mercer,Thomas M Badger

PloS one 13:e0196862 PubMed29723293

2018

Elevated androstenedione in young adult but not early adolescent prenatally androgenized female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ami B Shah,Isaac Nivar,Diana L Speelman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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