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AB136934

Angiotensin I ELISA kit

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Angiotensin I ELISA kit is a ELISA for the measurement of Angiotensin I in Human in Biofluids samples.

View Alternative Names

SERPINA8, AGT, Angiotensinogen, Serpin A8

1 Images
Competitive ELISA - Angiotensin I ELISA kit (AB136934)
  • cELISA

Supplier Data

Competitive ELISA - Angiotensin I ELISA kit (AB136934)

Representative standard curve using ab136934.

Key facts

Sample types

Plasma, Serum

Reacts with

Human

Sensitivity

= 4.3 pg/mL

Range

3.9 - 10000 pg/mL

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Precision

[ { "reproducibilityType": "Inter", "sample": "AGT", "replicates": 0, "mean": "24.4 pg/mL", "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Inter", "sample": "AGT", "replicates": 0, "mean": "48.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Inter", "sample": "AGT", "replicates": 0, "mean": "182.2 pg/mL", "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "AGT", "replicates": 20, "mean": "25.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "AGT", "replicates": 20, "mean": "53.2 pg/mL", "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "AGT", "replicates": 20, "mean": "163.6 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.3" } ]
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Recovery

[ { "sample": "Nasal Lavage Supernatant", "range": "76 - 90 %", "average": "= 77.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab136934-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB136934-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB136934-CMP10", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB136934-CMP05", "productcode":"" }, { "size":"1 x 4 mL", "name":"Angiotensin I Polyclonal Antibody", "number":"AB136934-CMP01", "productcode":"" }, { "size":"1 x 4 mL", "name":"Angiotensin I Biotinylated Conjugate", "number":"AB136934-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Buffer 16", "number":"AB136934-CMP04", "productcode":"" }, { "size":"1 x 1 µg", "name":"Angiotensin I Standard", "number":"AB136934-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB136934-CMP06", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"HRP- Streptavidin Conjugate", "number":"AB136934-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB136934-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensin I also known as Ang I acts as a precursor in the renin-angiotensin system. Its molecular mass is approximately 1296.5 Da. Angiotensin I originates from the cleavage of angiotensinogen by the enzyme renin primarily in the liver. This decapeptide circulates in the bloodstream and serves as a substrate for further enzymatic reactions. Although it itself does not have known biological activity its conversion to angiotensin II enhances its significance.
Biological function summary

The conversion of angiotensin I into angiotensin II is an important step. This reaction occurs in the lungs facilitated by the angiotensin-converting enzyme (ACE). Angiotensin II has much greater activity and plays an important role in increasing blood pressure and regulating blood volume. Angiotensin I serves as an important precursor member but not an active component of this system and enemies an intricate balance with other components like prorenin and renin.

Pathways

Angiotensin I's role is central in the renin-angiotensin-aldosterone system (RAAS). This pathway involves the conversion sequence involving renin Ang I ACE and angiotensin II leading further to the release of aldosterone. Angiotensin I plays a continuous part alongside other proteins such as ACE and angiotensinogen that integrate into the larger RAAS highlighting its intermediary function within this cardiovascular regulatory system.

Angiotensin I indirectly connects to hypertension and heart failure through its conversion to angiotensin II. Elevated levels of angiotensin II the main active agent can contribute to increased blood pressure and cardiovascular strain. The connection between angiotensin I and ACE accentuates its role in this pathological process even though Ang I itself is not directly pathogenic. Understanding this relationship provides insights into hypertension treatment strategies focusing on targeting ACE or using angiotensin I ELISA kits to monitor systemic levels.
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed : 10619573, PubMed : 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed : 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
See full target information AGT
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websiteProtocolBooklet
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