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AB302760

ARID1A ELISA Kit

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ARID1A ELISA Kit is a single-wash 90-min Simplestep used to quantify ARID1A with a sensitivity of 40.932 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BAF250, BAF250A, C1orf4, OSA1, SMARCF1, ARID1A, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, hOSA1

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Sandwich ELISA - ARID1A ELISA Kit (AB302760)
  • sELISA

Supplier Data

Sandwich ELISA - ARID1A ELISA Kit (AB302760)

Interpolated concentration of native ARID1A was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MCF-7 extract 200 µg/mL, C2C12 extract 500 µg/mL, C6 extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - ARID1A ELISA Kit (AB302760)
  • sELISA

Supplier Data

Sandwich ELISA - ARID1A ELISA Kit (AB302760)

Example of ARID1A standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human, Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40.932 pg/mL

Range

1718.75 - 110000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ARID1A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ARID1A protein in cell extracts. Quantitate ARID1A with 273 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "85 - 114 %", "average": "= 103" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab302760-96Tests", "assetComponentDetails": [ { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB302760-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB302760-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB302760-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB302760-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB302760-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB302760-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"ARID1A Lyophilized Recombinant Protein", "number":"AB302760-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X ARID1A Capture Antibody", "number":"AB302760-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB302760-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X ARID1A Detector Antibody", "number":"AB302760-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB302760-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.
Biological function summary

ARID1A impacts cellular functions through its role in the SWI/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.

Pathways

ARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.

Mutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.
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Product protocols

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information ARID1A
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Product promise

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