AB324920

BDNF ELISA Kit - Intracellular

SimpleStep
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BDNF ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify BDNF with a sensitivity of 3.6pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Brain-derived neurotrophic factor, Bdnf

5 Images

Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

sELISA

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Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

Interpolated concentration of native BDNF was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL human hippocampus tissue extract, mouse brain tissue extract, and rat cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

Recombinant prepro-BDNF, pro-BDNF, and mature BDNF proteins were tested for reactivity in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed. The kit reacts with both mature and pro-BDNF but weakly detects prepro-BDNF.

sELISA

Lab

Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

Example of BDNF standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

Interpolated concentration of native BDNF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL mouse brain tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - BDNF ELISA Kit - Intracellular (AB324920)

Example of BDNF standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.6 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

BDNF ELISA Kit- Intracellular (ab324920) is a rapid single-wash sandwich 90-min ELISA to measure BDNF in Tissue Extract. This SimpleStep sensitivity is 3.6 pg/ml.

How the assay works

BDNF ELISA Kit- Intracellular (ab324920) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

BDNF (ab324920) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our BDNF ELISA Kit- Intracellular only requires 12.5µL sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "101 - 119 %", "average": "= 107" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324920-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB324920-CMP13" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324920-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324920-CMP04" }, { "size":"10 x 600 µL", "name":"10X BDNF Detector Antibody", "number":"AB324920-CMP02" }, { "size":"10 x 600 µL", "name":"10X BDNF Capture Antibody", "number":"AB324920-CMP01" }, { "size":"20 x 1 Vial", "name":"BDNF Lyophilized Recombinant Protein", "number":"AB324920-CMP03" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324920-CMP10" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324920-CMP05" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324920-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324920-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324920-CMP07" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab324920-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB324920-CMP13" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324920-CMP04" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324920-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB324920-CMP09" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB324920-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB324920-CMP12" }, { "size":"1 x 600 µL", "name":"10X BDNF Detector Antibody", "number":"AB324920-CMP02" }, { "size":"1 x 600 µL", "name":"10X BDNF Capture Antibody", "number":"AB324920-CMP01" }, { "size":"2 x 1 Vial", "name":"BDNF Lyophilized Recombinant Protein", "number":"AB324920-CMP03" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324920-CMP06" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324920-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324920-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB324920-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324920-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324920-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324920-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324920-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324920-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324920-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324920-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324920-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"BDNF Lyophilized Recombinant Protein", "number":"AB324920-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X BDNF Capture Antibody", "number":"AB324920-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X BDNF Detector Antibody", "number":"AB324920-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity).. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.

See full target information Bdnf

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websiteProtocolBooklet

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Product promise

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