Anti-Bordetella pertussis toxin IgG ELISA Kit (PT) is an indirect ELISA for the qualitative detection of IgG class antibodies against Bordetella pertussis toxin (PT) in human plasma and serum samples.
- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
Images
Publications
Filter by
- Vaccine 39:1818-18252021Heterologous boosting of nonrelated toxoid immunity during acute Puumala hantavirus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiitus Lamponen,Iivo Hetemäki,Heikki J Niemi,Hanna Jarva,Eliisa Kekäläinen,Satu Mäkelä,Jukka Mustonen,Antti Vaheri,T Petteri ArstilaPubMed 33678453
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum
- Assay type
- Indirect
- Reactive species
- Human
- Range
- 25 - 200 IU/mL
- Sensitivity
- < 5 IU/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
What's included?
Recommended products
Anti-Bordetella pertussis toxin IgG ELISA Kit (PT) is an indirect ELISA for the qualitative detection of IgG class antibodies against Bordetella pertussis toxin (PT) in human plasma and serum samples.
- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum
- Assay type
- Indirect
- Reactive species
- Human
- Range
- 25 - 200 IU/mL
- Assay Platform
- Microplate
- Sensitivity
- < 5 IU/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 23 | C.V. 3.7 |
Sample Serum | n 24 | C.V. 2.2 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's anti-Bordetella pertussis toxin (PT) IgG in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies to Bordetella pertussis toxin (PT) in Human serum and plasma (citrate).
A 96-well plate has been precoated with Bordetella pertussis toxin to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized B. pertussis toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bordetella pertussis IgG commonly referred to as pertussis IgG is an immunoglobulin that targets the Bordetella pertussis bacterium the causative agent of whooping cough. This IgG specifically binds to the Bordetella pertussis toxin neutralizing its effects and helping prevent colonization and infection in the respiratory tract. Immunoglobulin G (IgG) is a major antibody type in the body's immune response and is found expressed in blood interstitial fluid and tissues throughout the body. Bordetella pertussis toxin a protein with a molecular mass of approximately 105 kDa plays a significant role in the pathogen's virulence.
- Biological function summary
The function of Bordetella pertussis IgG involves the human adaptive immune response where it aids in recognizing and neutralizing foreign pathogens like pertussis toxin. This IgG antibody does not form part of a larger complex itself but plays a critical role in the collective immune strategy. By binding with high affinity to the pertussis toxin the antibody blocks its interaction with host cells a step integral for the toxin’s ability to interfere with cellular processes and harm the host.
- Pathways
Several components including Bordetella pertussis IgG play a role in the immune recognition and response pathways specifically the humoral immune response. This antibody works in conjunction with other immune proteins like memory B cells and helper T cells which aid in generating a stronger response upon future exposure to the pathogen. The action of Bordetella pertussis IgG is essential in the adaptive immune pathway reinforcing the overall defense mechanism against such bacterial pathogens.
- Associated diseases and disorders
Bordetella pertussis IgG is most closely associated with whooping cough a highly contagious respiratory disease. The presence and levels of this IgG indicate immunity level against the disease whether from vaccination or past infection. Furthermore insufficient levels of this antibody can leave individuals susceptible to Bordetella pertussis infections. Pertussis toxin and its antibody Bordetella pertussis IgG serve as key components in diagnostics and understanding the disease's clinical progression and immune evasion strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Indirect ELISA - Anti-Bordetella pertussis toxin IgG ELISA Kit (PT) (ab178634)
Representative standard curve using ab178634.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com