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AB205069

Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit

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(1 Publication)

Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a Sandwich ELISA for the measurement of Bovine Alpha 1 Acid Glycoprotein / AGP in Cow in Biofluids samples.
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Sandwich ELISA - Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB205069)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB205069)

Representative standard curve using ab205069 Bovine Alpha 1 Acid Glycoprotein / AGP ELISA kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.706 ng/mL

Range

3.125 - 100 ng/mL

Assay time

1h 40m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alpha 1 Acid Glycoprotein / AGP in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP protein in bovine biological fluids.

In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with bovineradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205069-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Diluent Concentrate", "number":"AB205069-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205069-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Conjugated Enzyme Antibody", "number":"AB205069-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205069-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205069-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Bovine Alpha 1 Acid Glycoprotein / AGP Calibrator (Lyophilized)", "number":"AB205069-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Microplate", "number":"AB205069-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Product protocols

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Target data

See full target information ORM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1091066 PubMed36793724

2023

Application of quantitative proteomics to discover biomarkers for tick resistance in cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Raza,Benjamin L Schulz,Amanda Nouwens,Muhammad Noman Naseem,Muhammad Kamran,Emily F Mantilla Valdivieso,Edward D Kerr,Constantin Constantinoiu,Nicholas N Jonsson,Peter James,Ala E Tabor
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websiteProtocolBooklet
en
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Product promise

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