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AB273200

Bovine CXCL9 ELISA Kit

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Bovine CXCL9 ELISA Kit is a Sandwich ELISA for the measurement of Bovine CXCL9 in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

C-X-C motif chemokine 9, Small-inducible cytokine B9, CXCL9

1 Images
ELISA - Bovine CXCL9 ELISA Kit (AB273200)
  • ELISA

Supplier Data

ELISA - Bovine CXCL9 ELISA Kit (AB273200)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 32 pg/mL

Range

32 - 8000 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB202961

Anti-CXCL9 antibody

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bovine CXCL9 ELISA Kit (ab273200) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine CXCL9 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for bovine CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-bovine CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 120 %", "average": "= 105.8" }, { "sample": "Plasma", "range": "70 - 135 %", "average": "= 94.17" }, { "sample": "Cell culture media", "range": "69 - 93 %", "average": "= 75.73" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273200-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273200-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine CXCL9 coated Microplate (12 x 8 wells)", "number":"AB273200-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273200-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine CXCL9 Standard (Lyophilized)", "number":"AB273200-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273200-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB273200-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273200-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine CXCL9 Antibody", "number":"AB273200-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
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Product protocols

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Target data

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3 (By similarity).
See full target information CXCL9
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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