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Bovine CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine CXCL9 in Cow in Plasma, Cell culture supernatant, Serum samples.
Alternative names=C-X-C motif chemokine 9, Small-inducible cytokine B9, CXCL9
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Cow
- Range
32 - 8000 ng/mL
- Sensitivity
= 32 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Bovine CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine CXCL9 in Cow in Plasma, Cell culture supernatant, Serum samples.
Alternative names=C-X-C motif chemokine 9, Small-inducible cytokine B9, CXCL9
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Cow
- Range
32 - 8000 ng/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 32 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105.8 | Range 90 - 120 % |
Sample type Plasma | Average % = 94.17 | Range 70 - 135 % |
Sample type Cell culture media | Average % = 75.73 | Range 69 - 93 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Bovine CXCL9 ELISA Kit (ab273200) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine CXCL9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-bovine CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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