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AB273191

Bovine IFN-alpha ELISA Kit

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Bovine IFN-alpha ELISA Kit is a Sandwich ELISA for the measurement of Bovine IFN-alpha in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

Interferon alpha-1

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Sandwich ELISA - Bovine IFN-alpha ELISA Kit (AB273191)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine IFN-alpha ELISA Kit (AB273191)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.12 ng/mL

Range

0.12 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bovine IFN-alpha ELISA Kit (ab273191) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IFN-alpha in serum (bovine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "108 - 134 %", "average": "= 120.6" }, { "sample": "Plasma", "range": "99 - 147 %", "average": "= 118.8" }, { "sample": "Cell culture media", "range": "123 - 149 %", "average": "= 135.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273191-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273191-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273191-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273191-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB273191-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273191-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine IFN-alpha Standard (Lyophilized)", "number":"AB273191-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine IFN-alpha coated Microplate (12 x 8 wells)", "number":"AB273191-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine IFN-alpha Antibody", "number":"AB273191-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information IFNA1_BOVIN
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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