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Bovine IgG ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IgG in Cow in Plasma, Cell culture supernatant, Milk, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Milk, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cow

Range

3.125 - 100 ng/mL

Assay time

50m

Sensitivity

= 1.245 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
100X HRP Conjugated Enzyme Antibody
1 x 150 µL
20X Diluent Concentration
1 x 50 mL
20X Wash Buffer Concentrate
1 x 50 mL
Anti-Bovine IgG ElISA Microplate
1 x 1 Unit
Bovine IgG Calibrator (Lyophilized)
1 x 1 Vial
Chromogen Substrate Solution
1 x 12 mL
Stop Solution
1 x 12 mL

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Bovine IgG ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IgG in Cow in Plasma, Cell culture supernatant, Milk, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Milk, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cow

Range

3.125 - 100 ng/mL

Assay time

50m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.245 ng/mL

General recovery

>= 85%

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

>= 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

IgG in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgG protein in bovine cell culture supernatant, milk, plasma and serum.

In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Bovine IgG ELISA Kit (ab205078), expandable thumbnail

    Sandwich ELISA - Bovine IgG ELISA Kit (ab205078)

    Representative standard curve using ab205078 Bovine IgG ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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