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AB273196

Bovine IL-1 alpha ELISA Kit

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Bovine IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Bovine IL-1 alpha in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 alpha, IL-1 alpha, IL1A

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ELISA - Bovine IL-1 alpha ELISA Kit (AB273196)
  • ELISA

Supplier Data

ELISA - Bovine IL-1 alpha ELISA Kit (AB273196)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich (quantitative)

Sensitivity

= 16 pg/mL

Range

16.38 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bovine IL-1 alpha ELISA Kit (ab273196) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-1 alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for bovine IL-1 alpha coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- bovine IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 104 %", "average": "= 94.68" }, { "sample": "Plasma", "range": "84 - 101 %", "average": "= 89.82" }, { "sample": "Cell culture media", "range": "108 - 119 %", "average": "= 112.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273196-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273196-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273196-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine IL-1 alpha coated Microplate (12 x 8 wells)", "number":"AB273196-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273196-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273196-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine IL-1 alpha Standard (Lyophilized)", "number":"AB273196-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273196-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine IL-1 alpha Antibody", "number":"AB273196-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
See full target information IL1A
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websiteProtocolBooklet
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