Bovine IL-18 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IL-18 in Cow in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 0.41 - 100 ng/mL
- Sensitivity
- = 0.43 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
IGIF, IL18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
What's included?
Recommended products
Bovine IL-18 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IL-18 in Cow in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 0.41 - 100 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.43 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 74.45 | Range 68 - 85 % |
Sample type Plasma | Average % = 70.23 | Range 69 - 72 % |
Sample type Cell culture media | Average % = 71.41 | Range 68 - 74 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Bovine IL-18 ELISA Kit (ab277387) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-18 in serum (Bovine IL-18 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-18 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
ELISA - Bovine IL-18 ELISA Kit (ab277387)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
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