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AB277388

Bovine IL-4 ELISA Kit

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(1 Publication)

Bovine IL-4 ELISA Kit is a Sandwich ELISA for the measurement of Bovine IL-4 in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

1 Images
ELISA - Bovine IL-4 ELISA Kit (AB277388)
  • ELISA

Supplier Data

ELISA - Bovine IL-4 ELISA Kit (AB277388)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

8 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bovine IL-4 ELISA Kit (ab277388) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-4 in serum (Bovine IL-4 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 141 %", "average": "= 127.3" }, { "sample": "Plasma", "range": "101 - 140 %", "average": "= 120.3" }, { "sample": "Cell culture media", "range": "83 - 117 %", "average": "= 97.79" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277388-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277388-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277388-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine IL-4 coated Microplate", "number":"AB277388-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB277388-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277388-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277388-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB277388-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine IL-4 Antibody", "number":"AB277388-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine IL-4 standard protein (Lyophilized)", "number":"AB277388-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 8:697789 PubMed34458353

2021

Plasma Metabolomics Reveals Pathogenesis of Retained Placenta in Dairy Cows.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqiong Li,Zhengwei Zhao,Yang Yu,Xiaojun Liang,Shengyi Wang,Lei Wang,Dongan Cui,Meizhou Huang
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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