Bovine IL-4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IL-4 in Cow in Plasma, Cell culture supernatant, Serum samples.
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- Frontiers in veterinary science 8:6977892021Plasma Metabolomics Reveals Pathogenesis of Retained Placenta in Dairy Cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqiong Li,Zhengwei Zhao,Yang Yu,Xiaojun Liang,Shengyi Wang,Lei Wang,Dongan Cui,Meizhou HuangPubMed 34458353
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 8 - 2000 pg/mL
- Sensitivity
- = 8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Alternative names
Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4
What's included?
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Bovine IL-4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Bovine IL-4 in Cow in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 8 - 2000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 127.3 | Range 105 - 141 % |
Sample type Plasma | Average % = 120.3 | Range 101 - 140 % |
Sample type Cell culture media | Average % = 97.79 | Range 83 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Bovine IL-4 ELISA Kit (ab277388) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-4 in serum (Bovine IL-4 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product promise
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1 product image
ELISA - Bovine IL-4 ELISA Kit (ab277388)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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