AB273188

Bovine Insulin ELISA Kit

Name: Bovine Insulin ELISA Kit (ab273188) | Abcam
Brand: Abcam Limited
SKU: AB273188
Price: 940 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Bovine Insulin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Bovine Insulin in in Biofluids, Cell Culture Media samples from a wide range of species.

View Alternative Names

Insulin, INS

1 Images

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

sELISA

Supplier Data

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Rat, Cow, Human, Pig

Assay type

Sandwich (quantitative)

Sensitivity

= 4.5 µlU/mL

Range

4.5 - 300 µlU/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Bovine Insulin ELISA Kit (ab273188) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine Insulin in serum (Bovine Insulin concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "72 - 114 %", "average": "= 90.59" }, { "sample": "Plasma", "range": "81 - 122 %", "average": "= 101.9" }, { "sample": "Cell culture media", "range": "87 - 140 %", "average": "= 113.1" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273188-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273188-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine Insulin coated Microplate (12 x 8 wells)", "number":"AB273188-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273188-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273188-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273188-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273188-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine Insulin Standard (Lyophilized)", "number":"AB273188-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273188-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine Insulin Antibody", "number":"AB273188-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

style

left-column

Product protocols

style

left-column

Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information INS

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:29 PubMed38302986

2024

Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Huang,Yuanbin Li,Zhuang Chen,Huiping Ou,Yanjiao Tan,Hui Lin

Advances in pharmacological and pharmaceutical sciences 2022:3776676 PubMed35445196

2022

Effects of the Antiobesity Drugs Aplex and Venera on Certain Biochemical and Physiological Indices in Obese Adult Male Albino Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan S A Elpasty,Eman G E Helal,Ashraf M M Algendy,Hany N Yousef

Journal of dairy science 105:4520-4533 PubMed35248377

2022

Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB and lysosomal dysfunction in dairy cows with clinical ketosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Fang,Xinwei Li,Shu Wang,Qianming Jiang,Juan J Loor,Xiuhuan Jiang,Lingxue Ju,Hao Yu,Taiyu Shen,Men Chen,Yuxiang Song,Zhe Wang,Xiliang Du,Guowen Liu

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Bovine Insulin ELISA Kit

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Product promise