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AB193683

Bovine TNF alpha ELISA Kit

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(1 Publication)

Bovine TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Bovine TNF alpha in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images
Sandwich ELISA - Bovine TNF alpha ELISA Kit (AB193683)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine TNF alpha ELISA Kit (AB193683)

Standard curve in Assay Diluent A using ab193683 TNF alpha Bovine ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Bovine TNF alpha ELISA Kit (AB193683)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine TNF alpha ELISA Kit (AB193683)

Standard curve in Assay Diluent B using ab193683 TNF alpha Bovine ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.1 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNF alpha Bovine ELISA Kit (ab193683) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine TNF alpha in serum, plasmas and cell culture supernatant.

This assay employs an antibody specific for bovine TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNF alpha present in the samples. The wells are washed and biotinylated anti-bovine TNF alpha antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNF alpha bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 96 %", "average": "= 84.2" }, { "sample": "Plasma", "range": "70 - 100 %", "average": "= 89.63" }, { "sample": "Cell culture media", "range": "92 - 131 %", "average": "= 109.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193683-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193683-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193683-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Bovine TNF-alpha Microplate (12 strips x 8 wells)", "number":"AB193683-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine TFN-alpha Standards (lyophilized)", "number":"AB193683-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193683-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Bovine TNF-alpha Detection Antibody", "number":"AB193683-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193683-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB193683-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193683-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 8:697789 PubMed34458353

2021

Plasma Metabolomics Reveals Pathogenesis of Retained Placenta in Dairy Cows.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqiong Li,Zhengwei Zhao,Yang Yu,Xiaojun Liang,Shengyi Wang,Lei Wang,Dongan Cui,Meizhou Huang
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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