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AB273194

Bovine VEGF-A ELISA Kit

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Bovine VEGF-A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Bovine VEGF-A in Cow in Biofluids, Cell Culture Media samples.

View Alternative Names

VEGF, VEGFA, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

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Sandwich ELISA - Bovine VEGF-A ELISA Kit (AB273194)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine VEGF-A ELISA Kit (AB273194)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

Assay type

Sandwich (quantitative)

Sensitivity

= 0.12 ng/mL

Range

0.12 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bovine VEGF-A ELISA Kit (ab273194) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine VEGF-A in serum (bovine VEGF-A concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 80 %", "average": "= 71.91" }, { "sample": "Plasma", "range": "68 - 77 %", "average": "= 70.95" }, { "sample": "Cell culture media", "range": "79 - 102 %", "average": "= 87.89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273194-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273194-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine VEGF-A Standard (Lyophilized)", "number":"AB273194-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine VEGF-A Antibody", "number":"AB273194-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB273194-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine VEGF-A coated Microplate (12 x 8 wells)", "number":"AB273194-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273194-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273194-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273194-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
See full target information VEGFA
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