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AB136936

Bradykinin ELISA kit

4

(2 Reviews)

|

(10 Publications)

Bradykinin ELISA kit is a competitive ELISA designed to quantify Bradykinin with a sensitivity of 24.8 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 11.7 - 30000 pg/ml

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

1 Images
Competitive ELISA - Bradykinin ELISA kit (AB136936)
  • cELISA

Supplier Data

Competitive ELISA - Bradykinin ELISA kit (AB136936)

Representative standard curve using ab136936.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Assay type

Competitive

Sensitivity

= 24.8 pg/mL

Range

11.7 - 30000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Bradykinin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the accurate quantitative measurement of Bradykinin in plasma, serum and urine.

A goat anti-Rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of Bradykinin conjugated to biotin, followed by a solution of polyclonal antibody to Bradykinin. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of Bradykinin captured in the plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "66.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 11.9" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "209.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 10.3" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 0, "mean": "700.4 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 15" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "695.4 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "208.7 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "73.7 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.9" } ]
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Recovery

[ { "sample": "Plasma", "range": "98 - 130 %", "average": "= 110" }, { "sample": "Urine", "range": "88 - 139 %", "average": "= 109" }, { "sample": "Serum", "range": "103 - 123 %", "average": "= 113" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab136936-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB136936-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB136936-CMP10", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB136936-CMP09", "productcode":"" }, { "size":"1 x 55 mL", "name":"Assay Buffer 16", "number":"AB136936-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Bradykinin Conjugate", "number":"AB136936-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Bradykinin Antibody", "number":"AB136936-CMP02", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"HRP- Streptavidin Conjugate", "number":"AB136936-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB136936-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bradykinin Standard", "number":"AB136936-CMP04", "productcode":"" }, { "size":"2 x 10 mL", "name":"TMB Substrate", "number":"AB136936-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bradykinin also known as bradykinin (BK) is a peptide that plays an important role in multiple physiological processes. Bradykinin consists of nine amino acids and typically has a molecular weight of around 1060 daltons. It is primarily expressed in endothelial cells and can be produced by blood plasma proteins in response to specific stimuli. Bradykinin exemplifies a mediator of inflammation and vasodilation contributing significantly to vascular permeability and lowering blood pressure.
Biological function summary

This peptide acts as an important modulator in inflammatory and pain processes. It forms part of the kallikrein-kinin system where it interacts with specific G-protein-coupled receptors known as bradykinin receptor B1 and B2. Bradykinin contributes to complex biological functions including the dilation of blood vessels smooth muscle contraction and activation of pain fiber nerve endings. This activity makes it critical for the body’s response to inflammation and injury.

Pathways

Bradykinin participates in the kallikrein-kinin system and the renin-angiotensin system. In the renin-angiotensin system bradykinin is related to angiotensin-converting enzyme (ACE) which degrades bradykinin and counters its vasodilatory effects. Bradykinin's role in inflammation and blood pressure regulation is linked closely to other vasoactive peptides and cytokines contributing to its importance in diverse physiological pathways.

Bradykinin is associated with conditions such as hereditary angioedema and chronic cough. In hereditary angioedema excessive bradykinin production leads to recurrent episodes of swelling due to its capacity to increase vascular permeability. The peptide also plays a role in inducing a chronic cough as an adverse reaction via its action on sensory neurons in the respiratory tract. This is often observed in patients using ACE inhibitors as the inhibition leads to increased levels of bradykinin.
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Product protocols

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
See full target information KNG1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

BJA open 14:100415 PubMed40529720

2025

Systemic role of orexin A, substance P, bradykinin, and DABK in severe COVID-19 and 2.5-yr follow-ups: an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrike Heinicke,Steven R Talbot,Filippos Thanasis,Elisabeth H Adam,Andreas von Knethen,Andrea U Steinbicker,Sebastian Zinn,Kai Zacharowski,Armin N Flinspach

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404342 PubMed39340833

2024

TRPC3/6 Channels Mediate Mechanical Pain Hypersensitivity via Enhancement of Nociceptor Excitability and of Spinal Synaptic Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Chuan Sun,Wen-Juan Han,Zhi-Wei Dou,Na Lu,Xu Wang,Fu-Dong Wang,Sui-Bin Ma,Zhi-Cheng Tian,Hang Xian,Wan-Neng Liu,Ying-Ying Liu,Wen-Bin Wu,Wen-Guang Chu,Huan Guo,Fei Wang,Hui Ding,Yuan-Ying Liu,Hui-Ren Tao,Marc Freichel,Lutz Birnbaumer,Zhen-Zhen Li,Rou-Gang Xie,Sheng-Xi Wu,Ceng Luo

British journal of pharmacology 181:3760-3778 PubMed38872396

2024

Factor XII and prekallikrein promote microvascular inflammation and psoriasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Zhang,Zengrong Chen,Junyan Guo,Qing Wan,Yingjie Zhang,Huihui Li,Haojie Rao,Jianfeng Yang,Pengfei Xu,Hong Chen,Miao Wang

Oxidative medicine and cellular longevity 2022:7920915 PubMed36338343

2022

Bradykinin and Galectin-3 in Survived and Deceased Patients with COVID-19 Pneumonia: An Increasingly Promising Biochemical Target.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Nikolic Turnic,Viseslav Popadic,Slobodan Klasnja,Ana Sekulic,Novica Nikolic,Vladimir Zivkovic,Nevena Jeremic,Marijana Andjic,Nevena Draginic,Ivan Srejovic,Jovana Jeremic,Marija Zdravkovic,Vladimir Jakovljevic

Frontiers in endocrinology 13:873727 PubMed35634493

2022

The Bradykinin System Contributes to the Regulation of Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Tian Ni,Wang-Sheng Wang,Yun Liu,Yi-Kai Lin,Fan Zhang,Wen-Jia Lei,Li-Jun Ling,Fan Pan,Ya-Nan Zhu,Meng-Die Li,Tao Duan,Ming Liu,Kang Sun

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:623-632 PubMed34018345

2021

Investigation of the role of miR-221 in diabetic peripheral neuropathy and related molecular mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaole Wu,Xiaoyu Wang,Yiyu Yin,Lei Zhu,Fengchao Zhang,Jianping Yang

Journal of burn care & research : official publication of the American Burn Association 40:893-899 PubMed31250897

2019

C1 Esterase Inhibitor Activity Is Reduced in the Acute Phase Following Burn Injury: A Prospective Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Matsuura,Akinori Osuka,Tomoya Hirose,Hiroshi Ogura,Masashi Ueyama,Takeshi Shimazu

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez

Molecular pain 13:1744806917704138 PubMed28381109

2017

Prostanoid-dependent spontaneous pain and PAR-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4.

Applications

Unspecified application

Species

Unspecified reactive species

Misa Ito,Kentaro Ono,Suzuro Hitomi,Tomotaka Nodai,Teppei Sago,Kiichiro Yamaguchi,Nozomu Harano,Kaori Gunnjigake,Ryuji Hosokawa,Tatsuo Kawamoto,Kiyotoshi Inenaga

PLoS neglected tropical diseases 10:e0004713 PubMed27167223

2016

Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp.

Applications

Unspecified application

Species

Unspecified reactive species

Mônica L Vieira,Clément Naudin,Matthias Mörgelin,Eliete C Romero,Ana Lucia T O Nascimento,Heiko Herwald
View all publications
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