JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB285245

BSA ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

BSA ELISA Kit is a sandwich ELISA designed to quantify BSA with a sensitivity of 6.25 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 400 ng/mL

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

1 Images
Sandwich ELISA - BSA ELISA Kit (AB285245)
  • sELISA

Supplier Data

Sandwich ELISA - BSA ELISA Kit (AB285245)

BSA ELISA Kit Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 6.25 ng/mL

Range

6.25 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

BSA ELISA Kit ab285245 is a sandwich ELISA to measure BSA in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids with a sensitivity of 6.25 ng/ml.

How the assay works

The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

BSA ELISA Kit ab285245 protocol summary

1. Add standards or samples to appropriate wells. Incubate
2. Wash
3. Add HRP conjugate reagent to all wells. Incubate
4. Wash
5. Add Chromogen solutions to each well. Incubate.
6. Add stop solution and read at 450 nm.

This product is manufactured by BioVision, an Abcam company and was previously called E4466 QuickDetect™ BSA (Human) ELISA Kit. E4466-100 is the same size as the 96 test size of ab285245.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" } ]
style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285245-96Test", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB285245-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Wash buffer", "number":"AB285245-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Standard Diluent", "number":"AB285245-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution B", "number":"AB285245-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution A", "number":"AB285245-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Special Diluent", "number":"AB285245-CMP07", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Standard (400 ng/ml)", "number":"AB285245-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"HRP-Conjugate Reagent", "number":"AB285245-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285245-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB285245-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
style
left-column

Product protocols

style
left-column

Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB190806

Human Apolipoprotein B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apolipoprotein B ELISA Kit (AB190806)

  • 4
  • 1
Assay Kits

AB274403

Human Apo-H ELISA Kit (Apolipoprotein H)

Recombinant|SimpleStep

Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (AB274403)

  • 0
  • 0
Assay Kits

AB234578

Human ALT ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com