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BSA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of BSA in Human in Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

6.25 - 400 ng/mL

Sensitivity

< 6.25 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

What's included?

96 Test
Components
20X Wash buffer
1 x 20 mL
Chromogen Solution A
1 x 6 mL
Chromogen Solution B
1 x 6 mL
HRP-Conjugate Reagent
1 x 6 mL
Micro ELISA Plate
1 x 1 Unit
Plate sealers
2 x 1 Unit
Special Diluent
1 x 6 mL
Standard (400 ng/ml)
1 x 0.6 mL
Standard Diluent
1 x 6 mL
Stop Solution
1 x 6 mL

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BSA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of BSA in Human in Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

6.25 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

< 6.25 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 15

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 15

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human BSA ELISA Kit ab285245 (previously called QuickDetect BSA (Human) ELISA Kit, E4466) is a sandwich ELISA assay for measuring the quantity of BSA in human serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

No significant cross-reactivity or interference between this analyte and its analogues was observed.

This product is manufactured by BioVision, an Abcam company and was previously called E4466 QuickDetect™ BSA (Human) ELISA Kit. E4466-100 is the same size as the 96 test size of ab285245.

Supplementary info

Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Activity summary

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Associated diseases and disorders

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human BSA ELISA Kit (ab285245), expandable thumbnail

    Sandwich ELISA - Human BSA ELISA Kit (ab285245)

    BSA ELISA Kit Standard Curve.

    Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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