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AB325037

Caffeine ELISA Kit - Extracellular

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Caffeine ELISA Kit - Extracellular is a Competitive ELISA for the measurement of Caffeine - Extracellular in in Biofluids, Cell Culture Media samples.

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A1AR, A1R, A3AR, A3R, AA1R_HUMAN, AA3R_HUMAN, ACEE, ACES_HUMAN, ACLS, AD 026, ADORA 1, ADORA 3, AIS, ANDR_HUMAN, AR, AR8, ARA3, ARACHE, Acetylcholinesterase, Adenosine A3 receptor, Adenosine receptor A1, Adenosine receptor A3, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease), Apoptosis related acetylcholinesterase, Atherosclerosis, susceptibility to, included, BXR, DHTR, DKFZp686N23123, DNA binding protein, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), ER, ER-alpha, ER-beta, ERR a, ERR-alpha, ERR1 protein, ERR1_HUMAN, ER[a], ER[b], ESR, ESR B, ESR BETA, ESR1_HUMAN, ESRA, ESRL 1, ESRR A, ESTR B, Eag-related protein 1, Era, Erb, Erb2, Estr, Estra, Estradiol Receptor alpha, Estradiol Receptor beta, Estradiol receptor, Estrogen Receptor 1, Estrogen Receptor 2, Estrogen receptor, Estrogen receptor 1 (alpha), Estrogen receptor 2 (ER beta), Estrogen receptor 2 ER beta, Estrogen receptor alpha, Estrogen receptor beta 4, Estrogen receptor related 1, Estrogen receptor-like 1, Estrogen resistance, included, Estrogen-related receptor alpha, Estrra, Ether a go go related potassium channel protein, Ether-a-go-go-related gene potassium channel 1, Ether-a-go-go-related protein 1, GCPS, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI family zinc finger 3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, GPCR 2, Glioma associated oncogene family zinc finger 3, H-ERG, HDL cholesterol, augmented response of, to hormone replacement, included, HUMARA, HYSP1, IMD42, KCNH2_HUMAN, KD, Kennedy disease (KD), Kv11.1, LQT 2, MGC129539, Myocardial infarction, susceptibility to, included, N-ACHE, NR1F3, NR1I2_HUMAN, NR3A1, NR3A2, NR3B1, NR3C4, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Nuclear receptor subfamily 1 group I member 2, Nuclear receptor subfamily 3 group A member 1, Nuclear receptor subfamily 3 group A member 2, Nuclear receptor subfamily 3 group B member 1, Nuclear receptor subfamily 3 group C member 4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), ONR 1, OTTHUMP00000017718, OTTHUMP00000017719, OTTHUMP00000215173, OTTHUMP00000215174, OTTHUMP00000215175, Oncogene GLI3, Orphan nuclear receptor PAR 1, Orphan nuclear receptor PXR, PAP A, PAPB, PAR, PAR q, PPD IV, PRR, Potassium channel HERG, Potassium voltage gated channel subfamily H (eag related) member 2, Potassium voltage-gated channel subfamily H member 2, Pregnane X receptor, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, RDC 7, RNESTROR, RORG_HUMAN, RP11 552M11.7, RZR GAMMA, RZRG, Retinoic acid binding receptor gamma, Retinoid-related orphan receptor-gamma, Ri, Rorc, SBMA, SMAX1, SQT1, SXR, Spinal and bulbar muscular atrophy, Spinal and bulbar muscular atrophy (SBMA), Steroid and xenobiotic receptor, Steroid hormone receptor ERR1, TFM, TGPCR1, TOR, Testicular Feminization (TFM), Transcriptional activator GLI3, Transcriptional repressor GLI3R, Voltage gated potassium channel, subfamily H, member 2, Voltage-gated potassium channel subunit Kv11.1, YT, YT blood group, Zinc finger protein GLI 3, androgen receptor splice variant 4b, bA552M11.5, eag homolog, estrogen receptor related receptor alpha, hERG-1, hERR1, pregnane X nuclear receptor variant 2

7 Images
Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Interpolated concentration of native Caffeine was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 Human serum, and 1 : 4,000 Human plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Cell Extraction Buffer SSW.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Example of ​Caffeine​ standard curve in Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffiene was spiked into the following Mouse samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are 25% Mouse serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffeine was spiked into the following Rat samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are 25% Rat serum, plasma (citrate), plasma (EDTA) and plasma (heparin) The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffeine was spiked into the following samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are as follows : Human low serum at 25% and cell culture media at 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Interpolated concentration of native Caffeine was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 Human saliva and 1 : 20,000 Human urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Cell Extraction Buffer SSW.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Serum of ten individual healthy Species male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Caffeine concentration was determined to be 98.5 ng/mL with a range of 0.2 – 355.6 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cell culture supernatant

Assay type

Competitive

Results type

Qualitative

Sensitivity

= 2.391 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

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Product details

Caffeine ELISA Kit- Extracellular (ab325037) is a rapid single-wash competitive 90-min ELISA to measure Caffeine in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cell Culture Supernatant. This SimpleStep sensitivity is 2.391 pg/ml.<br><br><strong>How the assay works</strong><br><br>Caffeine ELISA Kit- Extracellular (ab325037) uses an affinity tag recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA® allows the formulation of the antibody-analyte complex in a single step, saving you significant time at the bench.<br>In the antigen-down format, the labeled antigen and the unlabeled antigen from your sample compete to bind to the HRP-tagged capture antibody. In the antibody-down format, the HRP-tagged antigen and the unlabeled antigen from your sample compete to bind to the capture antibody. The component labeled with the CaptSure™ affinity tag, either antigen or antibody, binds to the SimpleStep ELISA® plate. A decrease in signal indicates the presence of antigen in the sample when compared to wells containing only the labeled antigen.<br><br><strong>Assay Specificity</strong><br><br>Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.<br>Please refer to our protocol booklet for more details.<br><br><strong>Caffeine (ab325037) protocol summary</strong><br><br>1. Mix : add samples / standards to the wells together with the Captsure conjugate solution and HRP Conjugate solution. Incubate 1 hr at room temperature<br>2. Wash<br>3. Add TMB development solution - incubate for 10 min<br>4. Add Stop solution<br>5. Read the results on a plate reader at 450 nm<br><br>

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]

Recovery

[ { "sample": "Serum", "range": "87 - 115 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "98 - 105 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "101 - 115 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "95 - 102 %", "average": "= 97" }, { "sample": "Saliva", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Urine", "range": "102 - 110 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "95 - 104 %", "average": "= 99" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

websiteProtocolBooklet
en

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