Calretinin ELISA Kit

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Sandwich ELISA - Calretinin ELISA Kit (AB233616)

Representative image of Calretinin ELISA Kit (ab233616)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Calretinin Capture Antibody

- 10X Calretinin Detector Antibody

- Calretinin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

• sELISA

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Sandwich ELISA - Calretinin ELISA Kit (AB233616)

Relative expression of calretinin in normal tissue extracts.

Calretinin expression in non-neural and non-malignant tissues are expected to be significantly lower than neural tissues. Interpolated concentrations of native Calretinin in human brain, heart, liver, and placenta extract samples. The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows : brain extract 3 μg/mL, heart, liver, and placenta extract 100 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

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Sandwich ELISA - Calretinin ELISA Kit (AB233616)

Interpolated concentrations of native Calretinin in human, mouse, and rat brain extract samples.

The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows : brain extract 12.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

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Sandwich ELISA - Calretinin ELISA Kit (AB233616)

Interpolated concentrations of native Calretinin in human breast milk samples.

The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calretinin concentration was determined to be 227 ng/mL in neat human breast milk.

• sELISA

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Sandwich ELISA - Calretinin ELISA Kit (AB233616)

Example of Calretinin standard curve in 1X Cell Extraction Buffer PTR.

The Calretinin standard curve was prepared as described in Section 10.