AB234585

cAMP Assay Kit (Competitive ELISA)

Name: cAMP Assay Kit (Competitive ELISA) (ab234585) | Abcam
Brand: Abcam Limited
SKU: AB234585
Price: 620 USD
Availability: InStock

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(7 Publications)

cAMP Assay Kit (Competitive ELISA) is a competitive ELISA used to quantify cAMP with a sensitivity of 0.01nM.

- Colorimetric competitive ELISA - works on any plate reader
- No need for acetylation step
- Ready-to-use Anti-cAMP Ab coated 96-well plate and HRP substrate Green Probe supplied

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Functional Studies - cAMP Assay Kit (Competitive ELISA) (AB234585)

FuncS

Supplier Data

Functional Studies - cAMP Assay Kit (Competitive ELISA) (AB234585)

The kit can detect as low as 0.1 nM cAMP in a 100 μL reaction volume at 405nm after incubation with Green Probe for 1 hour (blue line) and 3 hours (red line).

FuncS

Supplier Data

Functional Studies - cAMP Assay Kit (Competitive ELISA) (AB234585)

The Absorbance can be read at 405 nm (blue line), 650 nm (red line) or 740 nm (Green line), the data are from the incubation with Green Probe for 3 hours.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture media, Plasma, Tissue Extracts, Urine

Assay type

Quantitative

Sensitivity

= 0.1 nM

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Product details

cAMP Assay Kit (Competitive ELISA) ab234585 is a competitive ELISA to measure cAMP Assay Kit (Competitive ELISA) in plasma, urine, cell culture media, cell lysate, tissue extracts with a sensitivity of 0.1 nM.

How the assay works:

Colorimetric ELISA cAMP Assay Kit (ab234585) is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test samples competes for anti-cAMP antibody with the HRPcAMP antibody conjugate, therefore inhibits the binding of HRPcAMP to anti-cAMP antibody. The kit provides the sensitive method for detecting adenylate cyclase activity in biochemical or cellbased assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step, and provides the readyto-use Anti-cAMP Ab coated 96-well plate and HRP substrate Green Probe to quantify the HRP activity. The color product formed is proportional to the activity of HRP-cAMP.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

cAMP Assay Kit (Competitive ELISA) ab234585 protocol summary

1. Add cAMP standard or test samples into the anti-cAMP coated 96-well plate and incubate at room temperature for 5-10 minutes
2. Add 1X HRP-cAMP conjugate and Incubate at room temperature for 3 hours
3. Wash wells 4 times with Washing Buffer. Add Green Probe and incubate at room temperature for 1 to 3 hours
4. Monitor absorbance increase at 405, 650 or 740 nm.

If you are using fluorometric detection, we recommend cAMP Assay Kit (Fluorometric) (ab138880).

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5037 PubMed39934157

2025

The antidiabetic effect of safflower yellow by regulating the GOAT/ghrelin/GHS-R1a/cAMP/TRPM2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiao Ma,Haifeng Zhang,Qihui Yan,Ping Wang,Weiying Guo,Lu Yu

Journal of neuroinflammation 21:329 PubMed39716241

2024

Prostanoid signaling in retinal cells elicits inflammatory responses relevant to early-stage diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Amy K Stark,John S Penn

Antioxidants (Basel, Switzerland) 13: PubMed38790690

2024

2-(4-Methylthiazol-5-yl) Ethyl Nitrate Hydrochloride Ameliorates Cognitive Impairment via Modulation of Oxidative Stress and Nuclear Factor Kappa B (NF-κB) Signaling Pathway in Chronic Cerebral Hypoperfusion-Associated Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Li,Shaofeng Xu,Ling Wang,Xiaoliang Wang

Journal of experimental & clinical cancer research : CR 43:105 PubMed38576043

2024

GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chaolong Yan,Zijiang Yang,Pin Chen,Yuyang Yeh,Chongjing Sun,Tao Xie,Wei Huang,Xiaobiao Zhang

Sexual medicine 9:100390 PubMed34246178

2021

Overexpressing miR-122-5p Inhibits the Relaxation of Vaginal Smooth Muscle in Female Sexual Arousal Disorder by Targeting Vasoactive Intestinal Peptide Receptor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Cong,Tao Gui,Qinchuan Shi,Jingjing Zhang,Jingyi Feng,Lianjun Pan,Jiehua Ma,Aixia Zhang

Evidence-based complementary and alternative medic 2021:8868527 PubMed33505510

2021

Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-B Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Bingfeng Lin,Zhifeng Li,Jie Su,Yulin Feng

Clinical genetics 97:712-722 PubMed32157680

2020

A paternally inherited non-sense variant c.424G>T (p.G142*) in the first exon of XLαs in an adult patient with hypophosphatemia and osteopetrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Chen,Yang Meng,Mengjia Tang,Yan Wang,Ying Xie,Shan Wan,Haoming Tian,Xijie Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

cAMP Assay Kit (Competitive ELISA)

Functional Studies - cAMP Assay Kit (Competitive ELISA) (AB234585)

Functional Studies - cAMP Assay Kit (Competitive ELISA) (AB234585)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

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Product promise