Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
cAMP ELISA Kit is a Competitive ELISA kit for the measurement of cAMP in Plasma, Serum, Other biological fluids samples.
Alternative names=3'-5'-cyclic adenosine monophosphate, Adenosine 3' 5' cyclic monophosphate, Adenosine 3閳 5閳 cyclic monophosphate, Cyclic adenosine monophosphate
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Other biological fluids
- Assay type
Competitive
- Range
1.56 - 100 ng/mL
- Sensitivity
= 0.94 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
cAMP ELISA Kit is a Competitive ELISA kit for the measurement of cAMP in Plasma, Serum, Other biological fluids samples.
Alternative names=3'-5'-cyclic adenosine monophosphate, Adenosine 3' 5' cyclic monophosphate, Adenosine 3閳 5閳 cyclic monophosphate, Cyclic adenosine monophosphate
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Other biological fluids
- Assay type
Competitive
- Range
1.56 - 100 ng/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.94 ng/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
cAMP ELISA kit (ab285319, E4715) uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com