cAMP ELISA Kit is a Competitive ELISA kit for the measurement of cAMP in Plasma, Serum, Other biological fluids samples.
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3'-5'-cyclic adenosine monophosphate, Adenosine 3' 5' cyclic monophosphate, Adenosine 3閳 5閳 cyclic monophosphate, Cyclic adenosine monophosphate
cAMP ELISA Kit is a Competitive ELISA kit for the measurement of cAMP in Plasma, Serum, Other biological fluids samples.
cAMP ELISA kit (ab285319, E4715) uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve
Cyclic adenosine monophosphate (cAMP) serves as an essential secondary messenger in various signal transduction pathways. Known also by its chemical abbreviation cAMP this small molecule has a mass of approximately 329.21 g/mol. cAMP is synthesized from ATP by the enzyme adenylate cyclase which is present in many cell types notably in neurons and various endocrine tissues. cAMP acts by activating protein kinase A (PKA) which then phosphorylates target proteins to alter cellular function.
CAMP regulates numerous cellular processes including metabolism gene expression and cell growth. It functions as an important part of G protein-coupled receptor (GPCR) signaling pathways where its concentration can significantly change influenced by external signals. cAMP modulates the activity of ion channels and various enzymes often participating in feedback loops to maintain cellular homeostasis.
CAMP integrates into critical signal transduction pathways such as the cyclic AMP-dependent pathway and the calcium signaling pathway. It works closely with proteins like CREB (cAMP response element-binding protein) to regulate gene transcription. cAMP also impacts other molecules like phosphodiesterases which break it down maintaining its transient nature in response to stimuli.
Irregular cAMP levels associate with pathologies like heart failure and certain types of cancer. Disruptions in cAMP signaling can lead to abnormal cell growth or inadequate cellular response to hormonal signals. Through its interaction with proteins like CFTR (cystic fibrosis transmembrane conductance regulator) cAMP influences disorders such as cystic fibrosis highlighting its critical role in maintaining cellular function and signaling balance.
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