cAMP ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify cAMP with a sensitivity of 0.23 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva
- Assay type
- Competitive
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.23 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
cAMP ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify cAMP with a sensitivity of 0.23 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva
- Assay type
- Competitive
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.23 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 8 | mean - | SD - | C.V. = 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 3 | mean - | SD - | C.V. = 2.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 106 | Range 99 - 116 % |
Sample type Citrate plasma | Average % = 110 | Range 106 - 116 % |
Sample type EDTA Plasma | Average % = 90 | Range 82 - 97 % |
Sample type Heparin Plasma | Average % = 94 | Range 93 - 95 % |
Sample type Urine | Average % = 105 | Range 102 - 108 % |
Sample type Cell culture media | Average % = 104 | Range 95 - 110 % |
Sample type Cerebral Spinal Fluid | Average % = 96 | Range 91 - 104 % |
Sample type Saliva | Average % = 111 | Range 102 - 118 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
cAMP SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of cAMP in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, and Saliva. Quantitate cAMP with 0.23 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Example of cAMP standard curve. OD (450 nm) values (mean +/- SD) are graphed.
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Serum from ten individual healthy Human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean cAMP concentration in detectable samples was determined to be 2.8 ng/mL with a range of 2.4 – 3.2 ng/mL
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Interpolated concentration of native cAMP was measured in duplicate at different sample concentrations. Undiluted samples are 2% Human urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin.) The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - cAMP ELISA Kit - Extracellular (ab323510)
Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Human saliva, 25% Bovine CSF, and 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Product protocols
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