AB323510

cAMP ELISA Kit - Extracellular

SimpleStep
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cAMP ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify cAMP with a sensitivity of 0.23 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Validated on a number of sample types including cerebrospinal fluid (CSF)

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Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Human saliva, 25% Bovine CSF, and 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Example of cAMP standard curve. OD (450 nm) values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Serum from ten individual healthy Human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean cAMP concentration in detectable samples was determined to be 2.8 ng/mL with a range of 2.4 – 3.2 ng/mL

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Interpolated concentration of native cAMP was measured in duplicate at different sample concentrations. Undiluted samples are 2% Human urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - cAMP ELISA Kit - Extracellular (AB323510)

Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin.) The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva

Assay type

Competitive

Sensitivity

= 0.23 ng/mL

Range

0.39 - 25 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

cAMP SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of cAMP in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, and Saliva. Quantitate cAMP with 0.23 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 116 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "106 - 116 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "82 - 97 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Urine", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "95 - 110 %", "average": "= 104" }, { "sample": "Cerebral Spinal Fluid", "range": "91 - 104 %", "average": "= 96" }, { "sample": "Saliva", "range": "102 - 118 %", "average": "= 111" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

See full target information Cyclic AMP

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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