cAMP ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify cAMP with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Competitive
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.22 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
cAMP ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify cAMP with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Competitive
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.22 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 8 | mean - | SD - | C.V. = 8.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. = 1.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 105 | Range 103 - 107 % |
Sample type Tissue Extracts | Average % = 98 | Range 96 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
cAMP SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of cAMP in Cell and Tissue extracts. Quantitate cAMP with 0.22 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - cAMP ELISA Kit - Intracellular (ab323511)
Free cAMP was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 0.5 mg/ HepG2 cell extract, Jurkat cell extract and Human kidney extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - cAMP ELISA Kit - Intracellular (ab323511)
Example of cAMP standard curve. OD (450 nm) values (mean +/- SD) are graphed.
Sandwich ELISA - cAMP ELISA Kit - Intracellular (ab323511)
Comparison of native cAMP concentration in treated versus control cell extract. HepG2 cells were treated with 30 µM Forskolin and 1 mM IBMX diluted in cell culture media for 15 minutes, scraped from the plate and then extracted with 1X Cell Extraction Buffer PTR. Mock control HepG2 cells accompanied cell treatment. Concentrations of cAMP were measured in duplicate and interpolated against the standard curve. Results show the mean cAMP concentration in control cell extract was 0.69 ng/mL compared to 11.20 ng/mL in the forskolin- and IBMX-treated sample.
Sandwich ELISA - cAMP ELISA Kit - Intracellular (ab323511)
Interpolated concentration of cAMP was measured in duplicate at different sample concentrations. Undiluted samples are 0.25 mg/mL HepG2 Cell Extract. Cells were treated for 15 minutes with 30 µM Forskolin and 1 mM IBMX. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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