AB277078

Canine Albumin ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Canine Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Canine Albumin with a sensitivity of 37.706 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Albumin, ALB

3 Images

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

sELISA

Supplier Data

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Interpolated concentrations of native Albumin in canine serum.

The concentrations of Albumin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 6,666,666. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 38.3 mg/mL in serum.

sELISA

Supplier Data

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Example of canine Albumin standard curve in Sample Diluent NS.

The Albumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Interpolated concentrations of native Albumin in canine urine.

The concentrations of Albumin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 12.2 μg/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Serum

Reacts with

Dog

Assay type

Sandwich (quantitative)

Sensitivity

= 37.706 pg/mL

Range

94 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Canine Albumin ELISA kit (ab277078) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Canine Albumin protein in canine serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Canine Albumin with 37.7 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Serum", "range": "108 - 119 %", "average": "= 113" }, { "sample": "Urine", "range": "109 - 120 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "81 - 92 %", "average": "= 88" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information ALB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e28420 PubMed38590903

2024

Evaluation of different media compositions promoting hepatocyte differentiation in the canine liver organoid model.

Applications

Unspecified application

Species

Unspecified reactive species

Vojtech Gabriel,Addison Lincoln,Christopher Zdyrski,Abigail Ralston,Hannah Wickham,Sydney Honold,Basant H Ahmed,Karel Paukner,Ryan Feauto,Maria M Merodio,Pablo Piñeyro,David Meyerholz,Karin Allenspach,Jonathan P Mochel

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Canine Albumin ELISA Kit

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Sandwich ELISA - Canine Albumin ELISA Kit (AB277078)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise