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AB273265

Canine BDNF ELISA Kit

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Canine BDNF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine BDNF in Dog in Biofluids, Cell Culture Media samples.

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Brain-derived neurotrophic factor, BDNF

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Sandwich ELISA - Canine BDNF ELISA Kit (AB273265)
  • sELISA

Supplier Data

Sandwich ELISA - Canine BDNF ELISA Kit (AB273265)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich (quantitative)

Sensitivity

= 0.5 pg/mL

Range

0.41 - 100 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine BDNF ELISA kit (ab273265) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine BDNF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Canine BDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of BDNF bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 105 %", "average": "= 97.51" }, { "sample": "Plasma", "range": "68 - 122 %", "average": "= 94.91" }, { "sample": "Cell culture media", "range": "88 - 91 %", "average": "= 89.66" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273265-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273265-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine BDNF coated Microplate (12 x 8 wells)", "number":"AB273265-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273265-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273265-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine BDNF Standard (Lyophilized)", "number":"AB273265-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine BDNF Antibody", "number":"AB273265-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273265-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273265-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity).
See full target information BDNF
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