Canine Cardiac Troponin I ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Canine Cardiac Troponin I - Intracellular in Dog, Monkey, Human in Tissue Extracts samples.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
TNNC1, TNNI3, Cardiac troponin I
Canine Cardiac Troponin I ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA kit for the measurement of Canine Cardiac Troponin I - Intracellular in Dog, Monkey, Human in Tissue Extracts samples.
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.2 |
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 11.1 |
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 99 | Range 85 - 115 % |
Canine Cardiac Troponin I - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I - Intracellular protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine Cardiac Troponin I - Intracellular with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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