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AB309307

Canine Cardiac Troponin I ELISA Kit - Intracellular

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Canine Cardiac Troponin I ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA for the measurement of Canine Cardiac Troponin I - Intracellular in Dog, Human, Monkey in Cell/Tissue Extracts samples.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

3 Images
Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)

Canine, human, and monkey heart tissue extract samples were measured in duplicate and interpolated using the canine protein standard. The interpolated values in ng per μg of tissue extract are plotted (mean +/- SD, n=2).

Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)

Interpolated concentration of native Cardiac Troponin I was measured in duplicate at different sample concentrations. Undiluted sample is 15 μg/mL heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Cardiac Troponin I ELISA Kit - Intracellular (AB309307)

Example of canine Cardiac Troponin I standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Dog, Monkey, Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.438 - 28 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Canine Cardiac Troponin I - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I - Intracellular protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine Cardiac Troponin I - Intracellular with 0.1 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" } ]

Recovery

[ { "sample": "Tissue Extracts", "range": "85 - 115 %", "average": "= 99" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3
websiteProtocolBooklet
en

Product promise

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