Canine CSF1R / MCSFR1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Canine CSF1R / MCSFR1 in Citrate plasma, Heparin Plasma, EDTA Plasma, Serum samples.
Colorimetric
Citrate plasma, Heparin Plasma, EDTA Plasma, Serum
Cell-based (quantitative)
Dog
25 - 1600 pg/mL
1h 30m
= 7.777 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Canine CSF1R / MCSFR1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Canine CSF1R / MCSFR1 in Citrate plasma, Heparin Plasma, EDTA Plasma, Serum samples.
Colorimetric
Citrate plasma, Heparin Plasma, EDTA Plasma, Serum
Cell-based (quantitative)
Dog
25 - 1600 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 7.777 pg/mL
97%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.73 |
Sample type | Average % | Range |
---|---|---|
Sample type Citrate plasma | Average % = 115 | Range 109 - 118 % |
Sample type Heparin Plasma | Average % = 115 | Range 109 - 118 % |
Sample type EDTA Plasma | Average % = 114 | Range 105 - 121 % |
Sample type Serum | Average % = 97 | Range 87 - 107 % |
Blue Ice
+4°C
+4°C
+4°C
Canine CSF1R / MCSFR1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF1R / MCSFR1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine CSF1R / MCSFR1 with 7.777 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Example of canine CSF1R/MCSFR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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