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Canine CSF1R / MCSFR1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Canine CSF1R / MCSFR1 in Citrate plasma, Heparin Plasma, EDTA Plasma, Serum samples.

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Images

Sandwich ELISA - Canine CSF1R / MCSFR1 ELISA Kit (AB309302), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, EDTA Plasma, Serum

Assay type

Cell-based (quantitative)

Reactive species

Dog

Range

25 - 1600 pg/mL

Assay time

1h 30m

Sensitivity

= 7.777 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).

Alternative names

What's included?

1 x 96 Tests
Components
10X Canine CSF1R / MCSFR1 Capture Antibody
1 x 600 µL
10X Canine CSF1R / MCSFR1 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 5BR
1 x 6 mL
Canine CSF1R / MCSFR1 Lyophilized Recombinant Protein
1 x 2 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 100 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Canine CSF1R / MCSFR1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Canine CSF1R / MCSFR1 in Citrate plasma, Heparin Plasma, EDTA Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, EDTA Plasma, Serum

Assay type

Cell-based (quantitative)

Reactive species

Dog

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 7.777 pg/mL

General recovery

97%

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

4

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

7.73

Recovery

Sample specific recovery

Sample type

Citrate plasma

Average %

= 115

Range

109 - 118 %

Sample type

Heparin Plasma

Average %

= 115

Range

109 - 118 %

Sample type

EDTA Plasma

Average %

= 114

Range

105 - 121 %

Sample type

Serum

Average %

= 97

Range

87 - 107 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Canine CSF1R / MCSFR1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF1R / MCSFR1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine CSF1R / MCSFR1 with 7.777 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Canine CSF1R / MCSFR1 ELISA Kit (ab309302), expandable thumbnail

    Sandwich ELISA - Canine CSF1R / MCSFR1 ELISA Kit (ab309302)

    Example of canine CSF1R/MCSFR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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