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AB273183

Canine CXCL1 ELISA Kit (GRO alpha)

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Canine CXCL1 ELISA Kit (GRO alpha) is a Sandwich ELISA for the measurement of Canine CXCL1 (GRO alpha) in Dog in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Canine CXCL1 ELISA Kit (GRO alpha) (AB273183)
  • sELISA

Supplier Data

Sandwich ELISA - Canine CXCL1 ELISA Kit (GRO alpha) (AB273183)

Canine CXCL1 ELISA Kit (GRO alpha) standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.4 pg/mL

Range

5.47 - 350 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine CXCL1 ELISA Kit (GRO alpha) (ab273183) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine CXCL1 (GRO alpha) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "121 - 145 %", "average": "= 134.8" }, { "sample": "Plasma", "range": "109 - 141 %", "average": "= 128.3" }, { "sample": "Cell culture media", "range": "108 - 149 %", "average": "= 128.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273183-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273183-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273183-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273183-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273183-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine CXCL1 coated Microplate (12 x 8 wells)", "number":"AB273183-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine CXCL1 Antibody", "number":"AB273183-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273183-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273183-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine CXCL1 Standard (Lyophilized)", "number":"AB273183-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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Product protocols

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Target data

See full target information CXCL1 Canine
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websiteProtocolBooklet
en
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Product promise

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