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AB273186

Canine Decorin ELISA Kit

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Canine Decorin ELISA Kit is a Sandwich ELISA for the measurement of Canine Decorin in Dog in Biofluids, Cell Culture Media samples.

View Alternative Names

DCN1C, DCN, Decorin, Bone proteoglycan II, PG-S2

1 Images
Sandwich ELISA - Canine Decorin ELISA Kit (AB273186)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Decorin ELISA Kit (AB273186)

Canine Decorin ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3 pg/mL

Range

2.46 - 600 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine Decorin ELISA Kit (ab273186) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Decorin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 143 %", "average": "= 124.1" }, { "sample": "Plasma", "range": "91 - 140 %", "average": "= 114.6" }, { "sample": "Cell culture media", "range": "100 - 125 %", "average": "= 107.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273186-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273186-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273186-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273186-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine Decorin coated Microplate (12 x 8 wells)", "number":"AB273186-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine Decorin Antibody", "number":"AB273186-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273186-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine Decorin Standard (Lyophilized)", "number":"AB273186-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273186-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May affect the rate of fibrils formation.
See full target information DCN
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com