Canine Decorin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Decorin in Dog in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 2.46 - 600 pg/mL
- Sensitivity
- = 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
DCN1C, DCN, Decorin, Bone proteoglycan II, PG-S2
What's included?
Recommended products
Canine Decorin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Decorin in Dog in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 2.46 - 600 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 124.1 | Range 103 - 143 % |
Sample type Plasma | Average % = 114.6 | Range 91 - 140 % |
Sample type Cell culture media | Average % = 107.7 | Range 100 - 125 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Canine Decorin ELISA Kit (ab273186) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Decorin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Terms & Conditions.
1 product image
Sandwich ELISA - Canine Decorin ELISA Kit (ab273186)
Canine Decorin ELISA Kit standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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