Canine Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Haptoglobin in Dog in Plasma, Serum samples.
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- Veterinary sciences 8:2021Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiacomo Rossi,Alessandra Gavazza,Silvia Vincenzetti,Sara Mangiaterra,Livio Galosi,Andrea Marchegiani,Graziano Pengo,Gianni Sagratini,Massimo Ricciutelli,Matteo CerquetellaPubMed 34679072
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 0.313 - 20 ng/mL
- Assay time
- 4h
- Sensitivity
- > 0.123 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Alternative names
Haptoglobin, HP
What's included?
Recommended products
Canine Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Haptoglobin in Dog in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 0.313 - 20 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- > 0.123 ng/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Haptoglobin Canine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit designed for the quantitative measurement of Haptoglobin levels in plasma, serum, and cell culture supernatants.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
- Biological function summary
Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.
- Pathways
The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.
- Associated diseases and disorders
Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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1 product image
Sandwich ELISA - Canine Haptoglobin ELISA Kit (ab137978)
Representative Standard Curve using ab137978
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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