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Canine Hemoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Hemoglobin in Dog in Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Dog

Range

6.25 - 200 ng/mL

Assay time

1h 40m

Sensitivity

= 3.107 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Involved in oxygen transport from the lung to the various peripheral tissues.

Alternative names

What's included?

1 x 96 Tests
Components
100X HRP Conjugated Enzyme Antibody
1 x 150 µL
20X Wash Buffer Concentrate
1 x 50 mL
5X Diluent Concentration
1 x 50 mL
Anti-Dog Hemoglobin ELISA Microplate
1 x 1 Unit
Chromogen Substrate Solution
1 x 12 mL
Dog Hemoglobin Calibrator (Lyophilized)
1 x 1 Vial
Stop Solution
1 x 12 mL

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Canine Hemoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Hemoglobin in Dog in Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Dog

Range

6.25 - 200 ng/mL

Assay time

1h 40m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3.107 ng/mL

General recovery

>= 85%

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

>= 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Hemoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemoglobin protein in dog plasma and serum.

In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity of Hemoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Supplementary info

Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Activity summary

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Associated diseases and disorders

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Canine Hemoglobin ELISA Kit (ab205071), expandable thumbnail

    Sandwich ELISA - Canine Hemoglobin ELISA Kit (ab205071)

    Representative standard curve using ab205071 Dog Hemoglobin ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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