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AB205071

Canine Hemoglobin ELISA Kit

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Canine Hemoglobin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine Hemoglobin in Dog in Biofluids samples.

View Alternative Names

Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain, HBA

1 Images
Sandwich ELISA - Canine Hemoglobin ELISA Kit (AB205071)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Hemoglobin ELISA Kit (AB205071)

Representative standard curve using ab205071 Dog Hemoglobin ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Dog

Assay type

Sandwich (quantitative)

Sensitivity

= 3.107 ng/mL

Range

6.25 - 200 ng/mL

Assay time

1h 40m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Hemoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemoglobin protein in dog plasma and serum.

In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity of Hemoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205071-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentration", "number":"AB205071-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205071-CMP06", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Conjugated Enzyme Antibody", "number":"AB205071-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205071-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205071-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Dog Hemoglobin Calibrator (Lyophilized)", "number":"AB205071-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Dog Hemoglobin ELISA Microplate", "number":"AB205071-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.
Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.
See full target information Hemoglobin subunit alpha
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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