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AB273185

Canine IFN-alpha ELISA Kit

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Canine IFN-alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine IFN-alpha in Dog in Cell Culture Media, Biofluids samples.

View Alternative Names

Interferon alpha-1/2, IFN-alpha-1/2

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Sandwich ELISA - Canine IFN-alpha ELISA Kit (AB273185)
  • sELISA

Supplier Data

Sandwich ELISA - Canine IFN-alpha ELISA Kit (AB273185)

Canine IFN-alpha ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Dog

Assay type

Sandwich (quantitative)

Sensitivity

= 0.18 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine IFN-alpha ELISA Kit (ab273185) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IFN-alpha in serum (Canine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA as an anti-coagulant. Citrate and heparin are not recommended as anti-coagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Canine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 109 %", "average": "= 100.9" }, { "sample": "Cell culture media", "range": "84 - 104 %", "average": "= 95.38" }, { "sample": "Plasma", "range": "91 - 106 %", "average": "= 98.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273185-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273185-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273185-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273185-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273185-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine IFN-alpha Antibody", "number":"AB273185-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IFN-alpha Standard (Lyophilized)", "number":"AB273185-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine IFN-alpha coated Microplate (12 x 8 wells)", "number":"AB273185-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273185-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information Interferon alpha-1/2
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websiteProtocolBooklet
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