Canine IFN-alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine IFN-alpha in Dog in Cell culture supernatant, Serum, EDTA Plasma samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 0.164 - 40 ng/mL
- Sensitivity
- = 0.18 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Alternative names
Interferon alpha-1/2, IFN-alpha-1/2
What's included?
Recommended products
Canine IFN-alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine IFN-alpha in Dog in Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 0.164 - 40 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.18 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100.9 | Range 91 - 109 % |
Sample type Cell culture media | Average % = 95.38 | Range 84 - 104 % |
Sample type Plasma | Average % = 98.4 | Range 91 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Canine IFN-alpha ELISA Kit (ab273185) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IFN-alpha in serum (Canine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA as an anti-coagulant. Citrate and heparin are not recommended as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Canine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Canine IFN-alpha ELISA Kit (ab273185)
Canine IFN-alpha ELISA Kit standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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