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AB273184

Canine IGFBP2 ELISA Kit

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Canine IGFBP2 ELISA Kit is a Sandwich ELISA for the measurement of Canine IGFBP2 in Dog in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Canine IGFBP2 ELISA Kit (AB273184)
  • sELISA

Supplier Data

Sandwich ELISA - Canine IGFBP2 ELISA Kit (AB273184)

Canine IGFBP2 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.055 ng/mL

Range

0.051 - 12.5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB188200

Anti-IGFBP2 antibody [EPR18012-257]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (AB188200)

  • 4
  • 1
Proteins & Peptides

AB63223

Recombinant human IGFBP2 protein

Bioactive

Western blot - Recombinant human IGFBP2 protein (AB63223)

  • 0
  • 0
Assay Kits

AB260064

Human C-Peptide ELISA Kit

Recombinant|SimpleStep

ELISA - Human C-Peptide ELISA Kit (AB260064)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine IGFBP2 ((Insulin-like Growth Factor Binding Protein 2) ELISA Kit (ab273184) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IGFBP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 112 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "91 - 104 %", "average": "= 98.77" }, { "sample": "Cell culture media", "range": "74 - 104 %", "average": "= 87.73" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273184-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273184-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"550X HRP-Streptavidin Concentrate", "number":"AB273184-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IGFBP2 Standard (Lyophilized)", "number":"AB273184-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine IGFBP2 coated Microplate (12 x 8 wells)", "number":"AB273184-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273184-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273184-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273184-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine IGFBP2 Antibody", "number":"AB273184-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information IGFBP2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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