AB157701
Canine IgG ELISA Kit is a sandwich ELISA designed to quantify Canine IgG with a sensitivity of 7.148 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 25 - 800 ng/mL
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 25 - 800 ng/mL
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Canine IgG ELISA Kit (AB157701)
Representative standard curve using ab157701 IgG Dog ELISA kit
Reactivity data
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Product details
Canine IgG ELISA Kit ab157701 is a sandwich ELISA to measure Canine IgG in serum, plasma, other biological fluids with a sensitivity of 7.148 ng/ml.
How the assay works
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Canine IgG ELISA Kit ab157701 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
How the assay works
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Canine IgG ELISA Kit ab157701 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
Precision
[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": null, "average": "> 85" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Journal of animal science and technology 65:550-561 PubMed37332283
2023
Effects of resistant starch on anti-obesity status and nutrient digestibility in dogs.
Applications
Unspecified application
Species
Unspecified reactive species
Hyun-Woo Cho,Kangmin Seo,Ju Lan Chun,Junghwan Jeon,Chan Ho Kim,Sejin Lim,Si Nae Cheon,Ki Hyun Kim
Veterinary world 16:834-843 PubMed37235154
2023
Influence of dietary supplementation with new strains on hematology, serum biochemistry, nutritional status, digestibility, enzyme activities, and immunity in dogs.
Applications
Unspecified application
Species
Unspecified reactive species
Kamonporn Panja,Sathita Areerat,Pipatpong Chundang,Pornsucha Palaseweenun,Nattaphong Akrimajirachoote,Jaruwan Sitdhipol,Punnathorn Thaveethaptaikul,Pennapa Chonpathompikunlert,Kanidta Niwasabutra,Pongsathon Phapugrangkul,Attawit Kovitvadhi
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