Canine IgG ELISA Kit

Canine IgG ELISA Kit (ab193768) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Canine IgG with 100 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

There are six classes of immunoglobulins in canine: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin in serum and is equally distributed in blood and tissue. In canine, the IgG class is further divided into four subclasses: IgG1, IgG2, IgG3, and IgG4. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y ike structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity.