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Canine IgM ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine IgM in Dog in Plasma, Serum, Other biological fluids samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Dog

Range

25 - 800 ng/mL

Sensitivity

= 4.756 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
100X HRP-conjugated anti-dog IgM antibody
1 x 150 µL
20X Wash Buffer Concentrate
1 x 50 mL
5X Diluent Concentrate
1 x 50 mL
Chromogen Substrate Solution
1 x 12 mL
Dog IgM Calibrator (Lyophilized)
1 x 1 Vial
Dog IgM ELISA Microplate
1 x 1 Unit
Stop Solution
1 x 12 mL

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Canine IgM ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine IgM in Dog in Plasma, Serum, Other biological fluids samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Dog

Range

25 - 800 ng/mL

Assay Platform

Microplate

Sensitivity

= 4.756 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

> 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's Dog IgM ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in dog serum and plasma samples.

In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Supplementary info

Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Activity summary

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

Associated diseases and disorders

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Canine IgM ELISA Kit (ab157702), expandable thumbnail

    Sandwich ELISA - Canine IgM ELISA Kit (ab157702)

    Representative standard curve using ab157702 IgM Dog ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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