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AB193685

Canine IL-10 ELISA Kit

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(1 Publication)

Canine IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Canine IL-10 in Dog in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
ELISA - Canine IL-10 ELISA Kit (AB193685)
  • ELISA

Supplier Data

ELISA - Canine IL-10 ELISA Kit (AB193685)

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.1 - 80 ng/well

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Canine ELISA Kit (ab193685) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-10 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for canine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-10 present in the samples. The wells are washed and biotinylated anti-canine IL-10 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-10 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 138 %", "average": "= 116.3" }, { "sample": "Plasma", "range": "93 - 149 %", "average": "= 120.1" }, { "sample": "Cell culture media", "range": "77 - 115 %", "average": "= 95.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193685-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193685-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB193685-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193685-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193685-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Canine IL-10 Detection Antibody", "number":"AB193685-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated canine IL-10 Microplate (12 strips by 8 wells)", "number":"AB193685-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IL-10 Standards (lyophilized)", "number":"AB193685-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193685-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin Concentrate", "number":"AB193685-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 9:1057972 PubMed36619946

2022

Curcuminoid supplementation in canine diabetic mellitus and its complications using proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Namphung Suemanotham,Pongsakorn Photcharatinnakorn,Boonrat Chantong,Shutipen Buranasinsup,Sataporn Phochantachinda,Walasinee Sakcamduang,Onrapak Reamtong,Tipparat Thiangtrongjit,Duangthip Chatchaisak
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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