AB273170
Canine IL-1beta ELISA Kit is a Sandwich ELISA for the measurement of Canine IL-1beta in Dog in Biofluids, Cell Culture Media samples.
View Alternative Names
Interleukin-1 beta, IL-1 beta, IL1B
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Canine IL-1beta ELISA Kit (AB273170)
caninine IL-1beta ELISA Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Canine IL-1beta ELISA Kit (ab273170) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for canine IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-canine IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for canine IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-canine IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": "74 - 98 %", "average": "= 86.02" }, { "sample": "Plasma", "range": "68 - 107 %", "average": "= 84.08" }, { "sample": "Cell culture media", "range": "109 - 145 %", "average": "= 126.1" } ]
What's included?
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273170-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273170-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine IL-1beta coated Microplate (12 x 8 wells)", "number":"AB273170-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273170-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IL-1beta Standard (Lyophilized)", "number":"AB273170-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273170-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine IL-1beta Antibody", "number":"AB273170-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273170-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273170-CMP06", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 24: PubMed37834419
2023
Effects of SKCPT on Osteoarthritis in Beagle Meniscectomy and Cranial Cruciate Ligament Transection Models.
Applications
Unspecified application
Species
Unspecified reactive species
Hye-Min Kim,Minseok Kang,Yoon-Seok Jung,Yoon-Jung Lee,Wonjae Choi,Hunseung Yoo,JeongHoon Kim,Hyo-Jin An
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