JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205084

Canine KIM-1 ELISA Kit

5

(1 Review)

|

(1 Publication)

Canine KIM-1 ELISA Kit is a Sandwich ELISA for the measurement of Canine KIM-1 in Dog in Biofluids samples.
1 Images
Sandwich ELISA - Canine KIM-1 ELISA Kit (AB205084)
  • sELISA

Supplier Data

Sandwich ELISA - Canine KIM-1 ELISA Kit (AB205084)

Representative standard curve using ab205084 Dog KIM-1 ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.034 ng/mL

Range

0.03125 - 1 ng/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB199083

Mouse Lipocalin-2 ELISA Kit (NGAL)

Recombinant|SimpleStep

ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

  • 5
  • 2
Primary Antibodies

AB78494

Anti-TIM 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 1 antibody (AB78494)

  • 3
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Kidney Injury Molecule-1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kidney Injury Molecule-1 protein in dog biological fluids.

In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205084-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205084-CMP08", "productcode":"" }, { "size":"1 x 60 mL", "name":"1X Diluent Concentrate", "number":"AB205084-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Streptavidin", "number":"AB205084-CMP06", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X Detection Antibody Conjugate", "number":"AB205084-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205084-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205084-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Dog KIM-1 Calibrator (Lyophilized)", "number":"AB205084-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Dog KIM-1 ELISA Microplate", "number":"AB205084-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.
style
left-column

Product protocols

style
left-column

Target data

See full target information HAVCR1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 14: PubMed39199847

2024

Early Detection of Chronic Kidney Disease Using Plasma Neutrophil Gelatinase-Associated Lipocalin and Kidney Injury Molecule-1 in Small-Breed Dogs: A Retrospective Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Sung Kim,Han-Jun Kim,Sun-Hee Do
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com