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AB309277

Canine Liver-FABP ELISA Kit

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Canine Liver-FABP ELISA Kit is a Sandwich ELISA for the measurement of Canine Liver-FABP in Dog in Biofluids samples.
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Sandwich ELISA - Canine Liver-FABP ELISA Kit (AB309277)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Liver-FABP ELISA Kit (AB309277)

Interpolated concentration of native Liver-FABP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 133, plasma (EDTA) 1 : 100, plasma (citrate) 1 : 20, and plasma (heparin) 1 : 20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Canine Liver-FABP ELISA Kit (AB309277)
  • sELISA

Supplier Data

Sandwich ELISA - Canine Liver-FABP ELISA Kit (AB309277)

Example of canine Liver-FABP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Urine

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.396 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB222517

Anti-liver FABP antibody [EPR20464]

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Primary Antibodies

AB207327

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine Liver-FABP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Liver-FABP protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine Liver-FABP with 5.396 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 122 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "101 - 118 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "99 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "102 - 126 %", "average": "= 117" }, { "sample": "Urine", "range": "87 - 99 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309277-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309277-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309277-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309277-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309277-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309277-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB309277-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine Liver-FABP Lyophilized Recombinant Protein", "number":"AB309277-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Canine Liver-FABP Capture Antibody", "number":"AB309277-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309277-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Canine Liver-FABP Detector Antibody", "number":"AB309277-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Dog L-FABP
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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