Canine Liver-FABP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Liver-FABP in Dog in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.396 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
Canine Liver-FABP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Liver-FABP in Dog in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.396 pg/mL
- General recovery
- 111%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 5.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 89 - 122 % |
Sample type EDTA Plasma | Average % = 108 | Range 101 - 118 % |
Sample type Heparin Plasma | Average % = 104 | Range 99 - 108 % |
Sample type Citrate plasma | Average % = 117 | Range 102 - 126 % |
Sample type Urine | Average % = 92 | Range 87 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Canine Liver-FABP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Liver-FABP protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Canine Liver-FABP with 5.396 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Canine Liver-FABP ELISA Kit (ab309277)
Interpolated concentration of native Liver-FABP was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:133, plasma (EDTA) 1:100, plasma (citrate) 1:20, and plasma (heparin) 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Canine Liver-FABP ELISA Kit (ab309277)
Example of canine Liver-FABP standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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