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Canine Osteopontin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Osteopontin in Dog in Urine, Plasma, Serum samples.
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Dog
- Range
0.156 - 10 ng/mL
- Assay time
2h 50m
- Sensitivity
= 0.07 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
Canine Osteopontin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine Osteopontin in Dog in Urine, Plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Dog
- Range
0.156 - 10 ng/mL
- Assay time
2h 50m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.07 ng/mL
- General recovery
> 85%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % >= 85 | Range |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Osteopontin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Osteopontin protein in dog plasma, serum and urine.
In this assay the Osteopontin present in samples reacts with the anti-Osteopontin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Osteopontin, is added and complexes are formed. Following a wash step, horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Osteopontin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Osteopontin in the test sample. The quantity of Osteopontin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Product protocols
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