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AB273171

Canine PDGF-BB ELISA Kit

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Canine PDGF-BB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine PDGF-BB in Dog in Biofluids, Cell Culture Media samples.

View Alternative Names

Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, PDGFB

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ELISA - Canine PDGF-BB ELISA Kit (AB273171)
  • ELISA

Supplier Data

ELISA - Canine PDGF-BB ELISA Kit (AB273171)

Canine PDGF-BB ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich (quantitative)

Sensitivity

= 4 pg/mL

Range

12.29 - 1200 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine PDGF-BB ELISA Kit (ab273171) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine PDGF-BB in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for canine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "134 - 148 %", "average": "= 141.6" }, { "sample": "Serum", "range": "122 - 147 %", "average": "= 132.2" }, { "sample": "Plasma", "range": "121 - 148 %", "average": "= 130.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273171-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273171-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine PDGF-BB Standard (Lyophilized)", "number":"AB273171-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB273171-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273171-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273171-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273171-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273171-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine PDGF-BB coated Microplate (12 x 8 wells)", "number":"AB273171-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine PDGF-BB Antibody", "number":"AB273171-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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