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AB273176

Canine TIMP2 ELISA Kit

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Canine TIMP2 ELISA Kit is a Sandwich ELISA for the measurement of Canine TIMP2 in Dog in Biofluids, Cell Culture Media samples.

View Alternative Names

Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

1 Images
Sandwich ELISA - Canine TIMP2 ELISA Kit (AB273176)
  • sELISA

Supplier Data

Sandwich ELISA - Canine TIMP2 ELISA Kit (AB273176)

Canine TIMP2 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Dog

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20 pg/mL

Range

20.48 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB1828

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Human IL-8 ELISA Kit

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Assay Kits

AB187394

Human TIMP1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP1 ELISA Kit (AB187394)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Canine TIMP2 ELISA Kit (ab273176) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine TIMP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 142 %", "average": "= 125.9" }, { "sample": "Plasma", "range": "107 - 141 %", "average": "= 120.4" }, { "sample": "Cell culture media", "range": "102 - 144 %", "average": "= 123.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273176-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273176-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273176-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine TIMP2 Antibody", "number":"AB273176-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273176-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine TIMP2 coated Microplate (12 x 8 wells)", "number":"AB273176-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273176-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine TIMP2 Standard (Lyophilized)", "number":"AB273176-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273176-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
See full target information TIMP2
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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