Canine TNF alpha ELISA Kit

Product details

Canine TNF alpha ELISA Kit ab193687 is a sandwich ELISA to measure Canine TNF alpha in serum, plasma, cell culture supernatant with a sensitivity of 2 pg/ml.

How the assay works

This assay employs an antibody specific for canine TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNF alpha present in the samples. The wells are washed and biotinylated anti-canine TNF alpha antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNF alpha bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Canine TNF alpha ELISA Kit ab193687 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.