Canine TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine TNF alpha in Dog in Plasma, Cell culture supernatant, Serum samples.
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- Journal of animal science and technology 65:550-5612023Effects of resistant starch on anti-obesity status and nutrient digestibility in dogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Woo Cho,Kangmin Seo,Ju Lan Chun,Junghwan Jeon,Chan Ho Kim,Sejin Lim,Si Nae Cheon,Ki Hyun KimPubMed 37332283
- The Journal of veterinary medical science 85:344-3572023Reconstruction of a partial esophageal defect using tunica vaginalis and buccal mucosa autograft: an experimental study in mongrel dogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed A Hashem,Elsayed Metwally,Yasmina K Mahmoud,Ibrahim E Helal,Mahmoud F AhmedPubMed 36709969
- Animal models and experimental medicine 4:268-2772021New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmir Abdolmaleki,Cyrus Jalili,Kamran Mansouri,Mitra BakhtiariPubMed 34557653
- International journal of medical sciences 17:1147-11552020N-acetylcysteine Restored Heart Rate Variability and Prevented Serious Adverse Events in Transfusion-dependent Thalassemia Patients: a Double-blind Single Center Randomized Controlled Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSintip Pattanakuhar et. al.PubMed 32547310
- Journal of veterinary pharmacology and therapeutic 42:258-2672019Effect of atorvastatin on oxidative stress and inflammation markers in myxomatous mitral valve disease in dogs: A comparison of subclinical and clinical stages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatchaya Thassakorn et. al.PubMed 30666669
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 2 - 700 pg/mL
- Sensitivity
- = 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
What's included?
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Canine TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Canine TNF alpha in Dog in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Dog
- Range
- 2 - 700 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101.1 | Range 93 - 109 % |
Sample type Plasma | Average % = 97.84 | Range 91 - 104 % |
Sample type Cell culture media | Average % = 118.4 | Range 110 - 126 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's TNF alpha Canine ELISA Kit (ab193687) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine TNF alpha in serum, plasma and cell culture supernatant.
This assay employs an antibody specific for canine TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNF alpha present in the samples. The wells are washed and biotinylated anti-canine TNF alpha antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNF alpha bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Canine TNF alpha ELISA Kit (ab193687)
Standard Curve in Assay Diluent A using ab193687 TNF alpha Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Sandwich ELISA - Canine TNF alpha ELISA Kit (ab193687)
Standard Curve in Assay Diluent B using ab193687 TNF alpha Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Downloads
Product protocols
- Visit the general protocols
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