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AB237648

Cetuximab ELISA Kit

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Cetuximab ELISA Kit is a sandwich ELISA designed to quantify Cetuximab with a sensitivity of 100 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 100 - 3000 ng/mL

View Alternative Names

Erbitux

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Sandwich ELISA - Cetuximab ELISA Kit (AB237648)
  • sELISA

Supplier Data

Sandwich ELISA - Cetuximab ELISA Kit (AB237648)

Example of Cetuximab standard curve.

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 100 ng/mL

Range

100 - 3000 ng/mL

Assay Platform

Microplate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Cetuximab ELISA Kit ab237648 is a sandwich ELISA to measure Cetuximab in serum, plasma with a sensitivity of 100 ng/ml.

How the assay works

Note: Cetuximab ELISA kit ab237648 is intended for Research Use Only, not for use in diagnostic procedures.

This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Cetuximab in the sample or standard. Results of samples can be determined directly using the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Cetuximab ELISA Kit ab237648 protocol summary

1. Add 100 μL of Assay buffer to each well and standard or sample to appropriate wells. Incubate at room temperature
2.Wash
3. Add HRP-conjugate to each well.Incubate at room temperature
4. Wash
5. Add TMB Substrate and incubate the plate in the dark at room temperature
6. Add Stop Solution and read OD at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4379 BioSim™ Cetuximab (Human) ELISA Kit. E4379-100 is the same size as the 96 test size of ab237648.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" } ]

Recovery

[ { "sample": "Serum", "range": "85 - 115 %", "average": null } ]

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab237648-96Test", "assetComponentDetails": [ { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S5", "number":"AB237648-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-conjugate Probe", "number":"AB237648-CMP02", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S4", "number":"AB237648-CMP06", "productcode":"" }, { "size":"2 x 50 mL", "name":"Assay Buffer", "number":"AB237648-CMP01", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S3", "number":"AB237648-CMP05", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S2", "number":"AB237648-CMP04", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S1", "number":"AB237648-CMP03", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S7", "number":"AB237648-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash buffer (20X)", "number":"AB237648-CMP12", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB237648-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB237648-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB substrate", "number":"AB237648-CMP13", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Cetuximab Standard S6", "number":"AB237648-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB237648-CMP14", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
websiteProtocolBooklet
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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